Abstract
Emphysema and chronic bronchitis which have different physiopathology, are the significant components of chronic obstructive lung disease (COPD). In emphysema models, it is known that bronchoalveloar lavage fluid (BALF) and matrix metalloproteinaz-9 (MMP-9) in serum are related with emphysema process. There is limited number of study claiming that adipose tissue-derived mesenchymal stem cells (MSC) obtained from have recovering effects on emphysema. The objective of the study is to investigate whether adipose tissue-derived MSCs have recovering effects on emphysema and on MMP-9 levels in an elastase rat model of emphysema.
In this study, 6/8- week-old 31 Wistar albino rats weighing between 250-300 g were assessed. In the first day of study, saline solution was administered intratracheally to the controls (n=10); 0.5 ml saline solution containing 0.1 IU "porcine" pancreatic elastase (PPE) per body weight in grams was administered intratracheally to the Elastase group (n=12) and the Elastase-MSC group (n=9). Rats in Elastase-MSC group were injected with MSCs suspended in serum physiologic via caudal vein at day 21. In 42nd day of study, all rats were sacrificed. BALF and serum MMP-9 concentrations were measured and emphysema index (EI) was calculated for both lung.
EI median values of Elastase (p=0.008) and Elastase-MSC (p=0.001) groups were significantly lower than that of control group. There was no statistically significant difference between serum and BALF MMP-9 levels of the groups (p>0.005 for two comparisons).
Our findings suggest that treatment with adipose tissue-derived MSC has no effect the serum and BALF MMP-9 levels in emphysematous rats.
- © 2014 ERS
