Abstract
Objective: To study the morphological features of the lung tissue in white rats with a model of asthma created by xenogeneic transplantation of adapted lymphocytes.
Methods: We intraperitoneally administered to 14 male albino rats washed lymphocytes from human patients with bronchial asthma in a dose of 5 000 000; 0.2 ml of 0.9% sodium chloride were intraperitoneally entered to the other 6 experimental rats. In 20 days after that autopsy was performed. Pieces of lung tissue were fixed in 10 % neutral formalin and embedded in paraffin. Paraffin sections (5-6 mkm thick) were stained with hematoxylin and eosin. Microscopic studies were conducted using light microscope.
Results: Macroscopically on autopsy lung tissue inflated with solitary hemorrhages under the visceral pleura. Lung tissue was pale pink, bronchial walls were thickened and deformed. We defined under the microscope emphysema, interspersed with areas of atelectasis, focal intraalveolar edema, spasm of the bronchial tubes, obstruction of the lumens of the bronchus and bronchioles by mucus and desquamated epithelium. Basal membrane and mucous of bronchioles was edematous and infiltrated by lymphocytes and eosinophils. Inflammatory infiltrate was detected in the submucosal layer. Peribronchial lung tissue was also infiltrated with eosinophils, lymphocytes. Areas of fibrinoid necrosis were identified.
In 6 intact control rats visible changes were not detected, i.e. development of experimental asthma has not happened.
Conclusion: The heterologous barrier is not an obstacle to transfer transplanted lymphocytes from asthmatic patients to rats for development of morphological changes that characterize asthma.
- © 2014 ERS