Abstract
The pseudomonas aeruginosa is a common opportunistic pathogen that can lead to death, especially to immunossupressed population, as elderly. Regular aerobic training (AT) improves lung response in pulmonary diseases and also the immune system. Therefore, this study investigated the possible protective effects of low intensity AT on the pulmonary inflammatory and immunological response in a model of acute lung injury induced by pseudomonas aeruginosa in elderly mice. The low intensity treadmill AT was performed during 4 weeks, 1 h/session, 4x/week in 24 weeks old C57Bl/6 male mice. Twenty-four hours after last AT session, mice were inoculated with 5 x 104 colony units of pseudomonas aeruginosa and were analyzed 24 hours after that. The results demonstrated that AT significantly reduced pseudomonas aeruginosa increased the numbers of total cells (p<0.01) and neutrophils (p<0.01) in the bronchoalveolar lavage (BAL) and the number of neutrophils in the lung parenchyma (p<0.01) when compared with pseudomonas group. The results also showed that AT reduced the levels of IL-1beta, IL-6, KC and TNF-alpha, while increased the levels of IL-10 in BAL (p<0.01) when compared with pseudomonas group. AT also increased the expression of IL-10 and reduced the expression of NF-kB in the lung when compared with pseudomonas group (p<0.01). In addtion, AT also increased the levels of catalase, glutatione peroxidase, superoxide dismutase and decreased the levels of lipoperoxidation in the lungs when compared with pseudomonas group (p<0.05). We conclude that AT inhibits acute lung injury induced by pseudomonas aeruginosa in ederly mice, through a IL-10/NF-kB/antioxidant-dependent mechanism.
- © 2014 ERS