Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions

Asthma and IgE associated polymorphisms influence TH17 pathway gene expression

Luise Worth, Sven Michel, Vincent Gaertner, Sebastian Kerzel, Michael Kabesch, Maximilian Schieck
European Respiratory Journal 2014 44: 3228; DOI:
Luise Worth
1Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sven Michel
1Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany
2Department of Pediatric Pneumology, Allergy and Neonatology, Hannover Medical School, Hannover, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vincent Gaertner
1Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastian Kerzel
1Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Kabesch
1Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany
2Department of Pediatric Pneumology, Allergy and Neonatology, Hannover Medical School, Hannover, Germany
3German Lung Research Center (DZL), German Lung Research Center, Hannover, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maximilian Schieck
1Department of Pediatric Pneumology and Allergy, University Children's Hospital Regensburg (KUNO), Regensburg, Germany
2Department of Pediatric Pneumology, Allergy and Neonatology, Hannover Medical School, Hannover, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Background: TH17 cells participate in the pathogenesis of autoimmune and inflammatory diseases, such as asthma. Recently, our group identified associations of asthma and total serum IgE levels with single nucleotide polymorphisms (SNPs) in TH17 pathway genes. We now investigated functional effects of these SNPs on gene expression within the TH17 pathway in vitro.

Methods: TH17 pathway SNPs were genotyped (23 by MALDI-TOF MS; 2 by RFLP) in a randomly selected cohort (n=57, German adults). Expression of ten signature TH17 pathway genes was measured by quantitative real-time PCR in peripheral blood mononuclear cells, stimulated with phytohemagglutinin, D. pteronyssinus, lipid A, or peptidoglycan. Induction of mRNA levels was compared between unstimulated and stimulated cells applying the delta-delta Ct method and correlated with genotypes. Measurements of TH17 pathway cytokines were performed.

Results: The RORyt SNP rs6693413, with minor allele frequency (MAF) 0.48, displayed allele-specific effects on IL17F mRNA induction in two of the tested stimulations (lowest p=0.013). Additionally, IL23A induction correlated with rs9395766 (IL17A; MAF=0.40) in all tested stimulations (lowest p=0.030).

Conclusion: Our results demonstrate that certain SNPs in the TH17 pathway are not only associated with asthma or IgE levels but also correlate with TH17 pathway gene expression. The observed effects of RORyt and IL17A variants on IL17F and IL23A expression support previous genetic studies, which suggested a central role of the IL-23/IL-17 axis within TH17 associated diseases.

  • Genetics
  • Functional genomics
  • Asthma - mechanism
  • © 2014 ERS
Previous
Back to top
Vol 44 Issue Suppl 58 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Asthma and IgE associated polymorphisms influence TH17 pathway gene expression
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
Asthma and IgE associated polymorphisms influence TH17 pathway gene expression
Luise Worth, Sven Michel, Vincent Gaertner, Sebastian Kerzel, Michael Kabesch, Maximilian Schieck
European Respiratory Journal Sep 2014, 44 (Suppl 58) 3228;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Asthma and IgE associated polymorphisms influence TH17 pathway gene expression
Luise Worth, Sven Michel, Vincent Gaertner, Sebastian Kerzel, Michael Kabesch, Maximilian Schieck
European Respiratory Journal Sep 2014, 44 (Suppl 58) 3228;
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Asthma phenotypes in children: Allergic versus non allergic
  • Analysis of risk factors in children with asthma exacerbations treated at the National Institute of Respiratory Diseases "Ismael Cosio Villegas" (INER)
  • Defining uncontrolled childhood asthma in the global PiCA consortium
Show more 7.2 Paediatric Asthma and Allergy

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • CME
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Submit a manuscript
  • ERS author centre

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2021 by the European Respiratory Society