Abstract
Introduction: Mesenchymal Stem Cells (MSCs) have been shown to contribute to pulmonary repair and regeneration in lung injury models. However, it is still unknown whether MSCs from patients with chronic diseases have the same regenerative / reparative capacity than MSCs from healthy subjects (H).
Aims: To study the functional and molecular response of bone marrow (BM)-MSCs and adipose derived (AD)-MSCs from patients with chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF) and H.
Methods: We have evaluated: 1) the cellular response of MSCs to VEGFs, PDFGs, tobacco; and, 2) the expression level of some of the associated growth factor receptors (GFR). MSCs were isolated from sternal aspirated BM samples and subcutaneous fat from subjects undergoing thoracic surgery. MSC functional response was real time monitored with the xCELLigence system. The amount of GFR was analyzed by RT-PCR and with LI-COR's Odyssey system. Tyrosine phosphorylation was checked by Western Blot.
Results: 1) MSCs from different origins respond to VEGF121, VEGF165, PDGFAA and PDFGBB indistinctive ways; 2) PDGFBB elicits the greatest response in tyrosine phosphorylation; 3) BM-COPD cells and ADSC-IPF cells have a significantly higher PDGFR beta expression than cells from H; and, 4) There seems to be a gradient response to tobacco stimulation, being MSC-COPD cells more sensitive than cells from other origins.
Conclusions: MSC-COPD and ADSC-IPF exhibit molecular and functional differences. Additional evaluation of these cells is needed to elucidate their potential involvement in the pathogenesis of these diseases.
Supported by PI10-00983, PI12-01152, SEPAR 2011 and 2013.
- © 2014 ERS
