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Effects of cigarette smoke extract on human airway smooth muscle cells in COPD

Ling Chen, Qi Ge, Gavin Tjin, Hatem Alkhouri, Linghong Deng, Corry-Anke Brandsma, Ian Adcock, Wim Timens, Dirkje Postma, Janette K. Burgess, Judith L. Black, Brian G.G. Oliver
European Respiratory Journal 2014 44: 634-646; DOI: 10.1183/09031936.00171313
Ling Chen
1Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Shapingba, Chongqing, China
2Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia
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Qi Ge
2Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia
3Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia
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Gavin Tjin
2Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia
4Central Clinical School, The University of Sydney, Sydney, NSW, Australia
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Hatem Alkhouri
5Respiratory Research Group, Faculty of Pharmacy, The University of Sydney, Sydney, NSW, Australia
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Linghong Deng
1Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Shapingba, Chongqing, China
6Institute of Biomedical Engineering and Health Science, Changzhou University, Changzhou, Jiangsu, China
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Corry-Anke Brandsma
7Dept of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
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Ian Adcock
8Thoracic Medicine, Imperial College London, National Heart and Lung Institute, London, UK
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Wim Timens
7Dept of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
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Dirkje Postma
9Dept of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
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Janette K. Burgess
2Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia
3Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia
4Central Clinical School, The University of Sydney, Sydney, NSW, Australia
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Judith L. Black
2Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia
3Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia
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Brian G.G. Oliver
2Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia
10School of Medical and Molecular Biosciences, University of Technology Sydney, Sydney, NSW, Australia
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  • For correspondence: brian.oliver@uts.edu.au
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Abstract

We hypothesised that the response to cigarette smoke in airway smooth muscle (ASM) cells from smokers with chronic obstructive pulmonary disease (COPD) would be intrinsically different from smokers without COPD, producing greater pro-inflammatory mediators and factors relating to airway remodelling.

ASM cells were obtained from smokers with or without COPD, and then stimulated with cigarette smoke extract (CSE) or transforming growth factor-β1. The production of chemokines and matrix metalloproteinases (MMPs) were measured by ELISA, and the deposition of collagens by extracellular matrix ELISA. The effects of CSE on cell attachment and wound healing were measured by toluidine blue attachment and cell tracker green wound healing assays.

CSE increased the release of CXCL8 and CXCL1 from human ASM cells, and cells from smokers with COPD produced more CSE-induced CXCL1. The production of MMP-1, -3 and -10, and the deposition of collagen VIII alpha 1 (COL8A1) were increased by CSE, especially in the COPD group which had higher production of MMP-1 and deposition of COL8A1. CSE decreased ASM cell attachment and wound healing in the COPD group only.

ASM cells from smokers with COPD were more sensitive to CSE stimulation, which may explain, in part, why some smokers develop COPD.

Abstract

Cigarette smoke extract induces airway remodelling-associated changes in airway smooth muscle cells in COPD patients http://ow.ly/wK0ey

Footnotes

  • This article has supplementary material available from erj.ersjournals.com

  • Conflict of interest: Disclosures can be found alongside the online version of this article at erj.ersjournals.com

  • Received October 1, 2013.
  • Accepted May 4, 2014.
  • ©ERS 2014
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Effects of cigarette smoke extract on human airway smooth muscle cells in COPD
Ling Chen, Qi Ge, Gavin Tjin, Hatem Alkhouri, Linghong Deng, Corry-Anke Brandsma, Ian Adcock, Wim Timens, Dirkje Postma, Janette K. Burgess, Judith L. Black, Brian G.G. Oliver
European Respiratory Journal Sep 2014, 44 (3) 634-646; DOI: 10.1183/09031936.00171313

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Effects of cigarette smoke extract on human airway smooth muscle cells in COPD
Ling Chen, Qi Ge, Gavin Tjin, Hatem Alkhouri, Linghong Deng, Corry-Anke Brandsma, Ian Adcock, Wim Timens, Dirkje Postma, Janette K. Burgess, Judith L. Black, Brian G.G. Oliver
European Respiratory Journal Sep 2014, 44 (3) 634-646; DOI: 10.1183/09031936.00171313
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