To the Editor:
Leptin, a product of the obese (ob) gene, was discovered as a hormone that plays a key role in regulating energy intake and expenditure. Over the past years, interest in the other functions of this pleiotropic hormone has increased. Leptin is primarily produced by adipocytes and is produced in lower amounts in other tissues. In the human lung, expression of leptin has been described in bronchial epithelial cells and alveolar macrophages [1]. Leptin receptors have a universal distribution that includes the respiratory system. The lung has mainly the Ob-Rb isoform, which is considered to be the fully functional receptor and most effective isoform [2]. Receptors have been identified in human bronchial and alveolar epithelial cells, bronchial smooth muscle cells, and bronchial submucosa [3]. The presence of these receptors together with evidence of local leptin production supports the concept that leptin may play a role in the regulation of airway diameter, lung development and the pathogenesis of respiratory diseases. The role of leptin in lung development has only been studied in animals and it is not known if these findings can be extended to the development of healthy human lungs. In this study we evaluated the relationship between plasma leptin levels and lung function in healthy young children and we assessed whether this relationship was modulated by the amount of adipose tissue deposits, the main source of leptin.
Participants in this study were children from the Wheezing Illness Study Leidsche Rijn (WHISTLER), an ongoing population-based birth cohort [4]. At the age of 8 years, participants were invited for a follow-up visit. During this visit, information on the child and parents was gathered by questionnaire and weight and height were measured. Spirometry was performed conforming to the latest American Thoracic Society/European Respiratory Society statements. Intra-abdominal and subcutaneous fat were measured using ultrasound [5]. After an overnight fast, a blood sample was taken in a sodium-heparin tube. A multiplex adipokine immunoassay, Luminex (Bio-Rad, Munich, Germany), was used for determination of the concentration of leptin [6]. This study was approved by the Medical Ethical Committee of the University Medical Center Utrecht (the Netherlands). Written informed parental consent was obtained.
Differences in subject characteristics above and below median leptin levels were tested using independent t-test (continuous variables) and Chi-squared (frequencies). The relationship between leptin (independent variable) and lung function (dependent variable) was assessed using linear regression analysis. Using multivariable linear regression we adjusted for possible confounders. Missing data on determinants were imputed using multiple imputations. Pooled results of 10 imputed datasets are expressed as linear regression coefficients with 95% confidence intervals. Due to a skewed distribution of residuals in the regression model with leptin, we entered leptin into the model after natural log transformation. To test if modification by sex or weight was statistically significant we added an interaction term (product of the potential effect modifier and leptin) to the regression model. All analyses were performed with SPSS version 20.0 for windows (IBM, Armonk, USA).
In total, 138 8-year-olds had successful spirometry and measurement of leptin concentration in plasma. Mean±sd forced expiratory volume in 1 s (FEV1) of these children was 1.78±0.27 L and the median (interquartile range) leptin level was 5.07 (1.80–7.40) ng·mL−1. The children with lower leptin levels were older (8.3 versus 8.0 years; p<0.01), had a lower weight (27.6 versus 28.6 kg; p<0.01) and a smaller waist circumference (57.5 versus 59.3 cm; p=0.03) than children with higher levels of leptin. No significant differences were observed in exposure to environmental factors.
Without adjustments, leptin was negatively associated with FEV1. Figure 1 shows the FEV1 plotted against leptin levels. The linear regression coefficient (95% CI) of the natural logarithm of leptin was -0.06 (-0.11– -0.01) L·ng−1·mL, which indicates that with a 10% increase in leptin, FEV1 decreases by 5.7 mL (i.e. by ln(1.1)×-0.06 L·ng−1·mL). Adjustment for age and sex slightly attenuated the association to -0.04 (-0.09–0.01) L·ng−1·mL and -0.05 (-0.10–0.00) L·ng−1·mL, respectively, while adjustment for anthropometrics strengthened the association. After adjustment for weight, a 10% increase in leptin was associated with an 11.4 mL decrease in FEV1. After adjustment for intra-abdominal and subcutaneous fat the association between leptin and FEV1 was still present (linear regression coefficient (95% CI) of -0.07 (-0.12–-0.02) L·ng−1·mL and -0.10 (-0.15– -0.06) L·ng−1·mL, respectively). The multivariable analyses with age, sex, weight and height and another model with breastfeeding, smoke exposure (during pregnancy and current), infections and allergies diagnosed by the general practitioner barely changed the linear regression coefficients of leptin compared with the univariable analysis. The regression analysis with forced vital capacity (FVC) as the dependent variable showed similar results. Weight and sex were not effect modifiers of the association (p-values for the interaction term of 0.28 and 0.96, respectively).
Forced expiratory volume in 1 s (FEV1) plotted against plasma leptin levels. The solid line is the regression line of the association between leptin and FEV1, dotted lines indicate 95% confidence intervals.
Previous studies of the role of leptin in lung development were all carried out in animal models, which represents a major limitation in extrapolating the results to humans. Animal studies have shown that leptin is important for postnatal development of the lungs. Genetically obese, leptin deficient mice (ob/ob mice) exhibit significantly lower lung volume and lower alveolar surface area at 2 weeks of age, when compared with heterozygotes or control animals [7]. Other evidence in mice has shown that leptin increases airway diameter [8]. The parasympathetic nervous system signals in airway smooth muscle cells to cause bronchoconstriction. The study showed that leptin signalling outside the hypothalamus in cholinergic neurons inhibited parasympathetic signalling in airway smooth muscle cells, which led to bronchodilation. In obese mice, bronchoconstriction occurred through leptin resistance [8]. Based on this data from animal studies one might expect that in healthy (nonobese) individuals higher leptin would lead to a more favourable lung function. However, we could not confirm this in our study involving young children, in which we observed the opposite relationship. The differences could be due to differences in autonomic nervous system innervations. Furthermore, mouse studies often reflect a model with either lipodystrophic “fat-free” or diet-induced obese mice in which leptin levels are either very low or high, which is not comparable with fat mass or leptin levels in our healthy group of children.
The direction of our findings is compatible with other studies performed in humans. A study performed in mainly asthmatic children showed a negative correlation between leptin and FEV1 % predicted and forced expiratory flow at 25–75% of FVC [9]. A large population-based study assessed the same association and found similar results in nonobese adults [10]. In our study, weight was not an effect-modifier in the association between leptin and lung function. As only few of our children were overweight, clinical leptin resistance was probably not present, although leptin resistance may gradually increase with increasing fat mass.
A nested case–control study in rescue workers, following the terrorist attacks on September 11, 2001 in New York City, NY, USA, showed that leptin was an independent risk factor for greater susceptibility to FEV1 impairment. Leptin increased the odds of abnormal FEV1 by more than twofold after adjustment for body mass index [11]. This longitudinal study supports the hypothesis that leptin has a direct effect on the airways, making reverse causation less likely.
As our study has a cross-sectional design, only assumptions about causality of associations can be made. A longitudinal study could give us more insights into the development of the respiratory system and the role of leptin. We measured the children at the age of 8 years. It would be interesting to measure children from an earlier age onwards. However, the lung grows until late adolescence and recent research showed that even alveolarisation is ongoing throughout childhood and adolescence in humans [12].
In conclusion, this is the first study to show an association between higher leptin plasma concentration and lower lung function, independent of adipose tissue in healthy children. The fact that we see such a strong association in healthy children supports the hypothesis that leptin has a functional role in the respiratory system.
Acknowledgments
The authors gratefully acknowledge all parents and children who participated, C. Geerts (Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, the Netherlands) and L. van der Feltz-Minkema (Dept of Paediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht) for their major contribution to the data collection, M. Olling-de Kok (Dept of Paediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht) for secretarial support, J. Zwerver (Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht) for data management and the Vascular Imaging Centre for assistance in the vascular measurements.
Footnotes
Support statement: The Wheezing Illnesses STudy LEidsche Rijn received grant 2100.0095 from The Netherlands Organisation for Health Research and Development (ZonMw). An unrestricted grant was received from GlaxoSmithKline. Additionally, this work was financially supported by the UMC Utrecht Vascular Prevention Project.
Conflict of interest: Disclosures can be found alongside the online version of this article at www.erj.ersjournals.com
- Received June 26, 2013.
- Accepted October 31, 2013.
- ©ERS 2014