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Conventional cytology vs liquid based cytology in preparation of bronchial wash, does the later have potential advantages?

Bhuvaneswari Kathiresan, Stephen Iles, Leonie Glinski
European Respiratory Journal 2013 42: P5091; DOI:
Bhuvaneswari Kathiresan
1Respiratory, Plymouth Hospitals NHS Trust, Plymouth, United Kingdom
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Stephen Iles
2Respiratory, Royal Cornwall NHS Trust, Truro, United Kingdom
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Leonie Glinski
3Pathology, Royal Cornwall NHS Trust, Truro, United Kingdom
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Abstract

Introduction

Liquid based cytology and Conventional cytology are two techniques used in preparation of bronchial washings and brushings.

Aims

To determine whether Liquid based cytology has better diagnostic rate compared to Conventional cytology when used in preparation of bronchial wash samples in patients with suspected lung cancer.

Methods

Retrospective observational study of bronchial wash samples sent for cytological analysis with radiological diagnosis of lung carcinoma between April 2009 and June 2012 in Royal Cornwall Hospital, United Kingdom.

Results

Conventional cytology was used in preparation of bronchial wash between April 2009 - July 2010 and Liquid based cytology between August 2010 – June 2012. 114 patients had their samples processed using Conventional cytology and 126 using Liquid based cytology during this period.

43 (38%) had positive cytology in Conventional group. 68 (54%) had positive cytology in Liquid based group. The calculated p-value is 0.017 (Two sample t test), suggesting statistically significant difference between two groups.

The volume of sample did not influence diagnostic yield, as the mean volume of sample in Liquid based group is same (7ml) in both positive and negative groups.

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Conclusion

Our study demonstrates improved diagnostic rate with Liquid based cytology, which might be due to preserving cells, removing excess red cells and eliminating spread artifact.

  • Thoracic oncology
  • Bronchoscopy
  • © 2013 ERS
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Conventional cytology vs liquid based cytology in preparation of bronchial wash, does the later have potential advantages?
Bhuvaneswari Kathiresan, Stephen Iles, Leonie Glinski
European Respiratory Journal Sep 2013, 42 (Suppl 57) P5091;

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Conventional cytology vs liquid based cytology in preparation of bronchial wash, does the later have potential advantages?
Bhuvaneswari Kathiresan, Stephen Iles, Leonie Glinski
European Respiratory Journal Sep 2013, 42 (Suppl 57) P5091;
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