Abstract
Introduction: Hemo2life is a new natural oxygen carrier extracted from Arenicola marina with high oxygen affinity acting at low temperature. We assessed the effect of Hemo2life associated with a static preservation solution on primary graft dysfunction after lung transplantation.
Material and Methodes: A left lung transplant was performed in pigs after 24 h of preservation at 4°C with Perfadex (Perfadex group, n=5) or with Perfadex® associated with Hemo2life (2g/l) (Hemo2life group, n=5) and compared to a sham animals (n=5). Expression of HIF1α was quantified on iterative samples from the right lung during preservation. During 5 h of lung reperfusion, hemodynamics, oxygenation and dynamic compliance were monitored. HMG-B1, TNFα, and NO were measured in serum. After 5 hours of reperfusion, TNFα and IL-8 were assayed in bronchoalveolar lavage (BAL).
Results : During cold ischemia, expression of HIF1 α and histology remained unchanged and similar to control. After 5 hours of reperfusion, Hemo2life group led to a significant reduction of graft vascular resistance (p<0.05), graft oxygenation ratio was significantly higher (p<0.05). Expression of HMG B1 in serum tended to be lower (2.1+/- 0.8 vs 4.6+/-1.5) compared with Perfadex group. TNF-alpha and IL-8 in BAL were significantly higher in the 2 experimental groups compared to control (p<0,05).
Conclusion : In this preliminary study, adjunction of a new oxygen carrier Hemo2life® in lung preservation solution improves early graft function after prolonged cold ischemia.
- © 2013 ERS
