Abstract
Dendritic cells induce and regulate T cell responses, and tolerogenic dendritic cells (DCs) can promote the development of regulatory T cells with suppressive activity. 1,25-dihydroxyvitamin D3 (1, 25(OH)2D3) is a secosteroid hormone that can cause tolerogenic dendritic cells (tDCs).However, the effect of tDCs activated by OVA on the production of interleukin (IL)-10 and IL-17 has not been well understood. We studied tDC effect on the production of IL-10 and IL-17 by using 1,25(OH)2D3-treated mouse bone marrow-derived immature dendritic cells (D3/imDC) exposed to ovalbumin (OVA)(OVA-D3/imDC) co-cultured with allogeneic naïve CD4+T cells in vitro. First, we identified the phenotypes of the OVA-D3/imDCs. Then the capacity of OVA-D3/imDCs production of IL-10 and IL-17 were verified through analysis of the production of IL-17 and IL-10 in co-culture supernatant. OVA-D3/imDCs displayed a semi-mature phenotype through up-regulation of CD86 and MHC II and inhibition of allogeneic CD4+T cells proliferations. OVA-D3/imDCs promoted IL-10 secretion and inhibited IL-2 secretion. Co-culture of OVA-D3/imDCs with naïve CD4+T cells induced CD4+Foxp3+Tregs. More importantly, OVA-D3/imDCs co-cultured with allogeneic naïve CD4+T cells decreased IL-17 levels in co-culture supernatant; However, OVA-D3/imDCs co-cultured with allogeneic naïve CD4+T cells enhanced IL-10 levels in co-culture supernatant. Our results suggested OVA-activated tolerogenic mouse bone marrow dendritic cells have ability to suppress the production of IL-17 and promote the production of IL-10.
- © 2013 ERS
