Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Controlled mechanical ventilation in septic animals: Effects on diaphragm function

Karen Maes, Debby Thomas, Cielen Nele, Smuder Asley, Powers Scott, Hermans Greet, Stamiris Angela, Sabah Hussain, Marc Decramer, Ghislaine Gayan-Ramirez
European Respiratory Journal 2013 42: 388; DOI:
Karen Maes
1Department of Clinical and Experimental Medicine, KULeuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Debby Thomas
1Department of Clinical and Experimental Medicine, KULeuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cielen Nele
1Department of Clinical and Experimental Medicine, KULeuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Smuder Asley
2Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Powers Scott
2Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hermans Greet
3Medical Intensive Care Unit, University Hospital, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stamiris Angela
4Department of Critical Care Medicine, McGill University, Montreal, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sabah Hussain
4Department of Critical Care Medicine, McGill University, Montreal, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marc Decramer
1Department of Clinical and Experimental Medicine, KULeuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ghislaine Gayan-Ramirez
1Department of Clinical and Experimental Medicine, KULeuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Controlled mechanical ventilation (CMV) was shown to result in diaphragmatic dysfunction and atrophy in healthy animals. Whether mechanical ventilation results in more severe diaphragm dysfunction in animals with already weakened diaphragms is not known and was examined in septic animals. Male Wistar rats were either treated with lipopolysaccharide (5 mg/kg) to induce sepsis or with saline. After 12 hours they were divided into 3 groups: sepsis, sepsis+12hCMV and saline+12hCMV. The levels of IL-6 in plasma and in diaphragm were significantly increased in the sepsis+12hCMV group compared to the other groups. Diaphragm force was significantly lower in septic animals compared to saline+12hCMV with an additional decrease in the sepsis+12hCMV group compared to the sepsis group. mRNA expression of diaphragm MuRF1 and atrogin-1, markers of the proteasome system, were significantly higher in the sepsis+12hCMV group compared to the other groups. Diaphragm fiber dimensions and 4-HNE, a marker of oxidative stress, were similar in all groups. LC3B protein lipidation, used as an indirect assessment of autophagy, was increased in sepsis+12hCMV compared to the other groups. Similarly, protein levels of several autophagy-related genes including those involved in the initiation of autophagosome formation (ULK1, BECLIN1, PI3KC3), and targeting of the mitochondria by autophagosomes (SQSTM1, BNIP3 and PARKIN) were significantly increased in sepsis+12hCMV compared to the others. These data clearly show that CMV in septic animals further compromises diaphragm function, activates atrophic pathway and favors autophagosome formation.

Supported by FWO-Flanders G.0893.11 and AstraZeneca Pharmaceuticals.

  • Mechanical ventilation
  • Sepsis
  • Animal models
  • © 2013 ERS
Previous
Back to top
Vol 42 Issue Suppl 57 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Controlled mechanical ventilation in septic animals: Effects on diaphragm function
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Controlled mechanical ventilation in septic animals: Effects on diaphragm function
Karen Maes, Debby Thomas, Cielen Nele, Smuder Asley, Powers Scott, Hermans Greet, Stamiris Angela, Sabah Hussain, Marc Decramer, Ghislaine Gayan-Ramirez
European Respiratory Journal Sep 2013, 42 (Suppl 57) 388;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Controlled mechanical ventilation in septic animals: Effects on diaphragm function
Karen Maes, Debby Thomas, Cielen Nele, Smuder Asley, Powers Scott, Hermans Greet, Stamiris Angela, Sabah Hussain, Marc Decramer, Ghislaine Gayan-Ramirez
European Respiratory Journal Sep 2013, 42 (Suppl 57) 388;
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
  • Info & Metrics
  • PDF
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • High-frequency chest wall oscillation assessment by electrical impedance tomography in intubated patients
  • Introducing the Homburg lung: Efficacy and safety of a minimal-invasive system for extracorporeal CO2 removal
  • Diaphragmatic ultrasonography during spontaneous breathing trials in critically ill patients: Can it identify weaning failure?
Show more 2.1 Acute Critical Care

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society