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Effect of cigarette smoke on peripheral blood Th17 (PBTh17) cells from COPD patients. Role of acetylcholine

Mirella Profita, Giusy Daniela Allbano, Caterina Di Sano, Angela Marina Montalbano, Rosalia Gagliardo, Giulia Anzalone, Loredana Riccobono, Anna Bonanno, Michael Paul Pieper, Mark Gjomarkaj
European Respiratory Journal 2012 40: P1427; DOI:
Mirella Profita
1IBIM, CNR, Palermo, Italy
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Giusy Daniela Allbano
1IBIM, CNR, Palermo, Italy
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Caterina Di Sano
1IBIM, CNR, Palermo, Italy
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Angela Marina Montalbano
1IBIM, CNR, Palermo, Italy
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Rosalia Gagliardo
1IBIM, CNR, Palermo, Italy
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Giulia Anzalone
1IBIM, CNR, Palermo, Italy
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Loredana Riccobono
1IBIM, CNR, Palermo, Italy
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Anna Bonanno
1IBIM, CNR, Palermo, Italy
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Michael Paul Pieper
2Boehringer Ingelheim, Pharma, Biberach, Germany
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Mark Gjomarkaj
1IBIM, CNR, Palermo, Italy
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Abstract

PBTh17 cells producing IL-17A and IL-22, as well as Acetylcholine (ACh) are involved in the systemic inflammation of several autoimmune and inflammatory diseases, including COPD.

We investigated the effect of cigarette smoke extract (CSE) and the role of ACh on the expression of RORgt, IL-17A and IL-22 in PBTh17 cells during systemic inflammation in COPD.

ACh, IL-17A, IL-22 and RORgt levels as well as the colocalization of ACh with IL-17A, IL-22 and RORgt were evaluated (flowcytometry) in PBT-lymphocytes (PBT) from COPD patients (n=16), healthy smokers (HS) (n=12) and healthy control subjects (HC) (n=13). Furthermore, PBT cells from COPD (n=6) patients and PBT cells from HC (n=6) stimulated in vitro with CSE (10 %) were cultured for 72 hrs in the presence or absence of Tiotropium (Spiriva®) (20 nM) and Olodaterol (1 nM) alone or in combination.

The colocalization of ACh with IL-17A, IL-22 and RORgt was significantly increased in PBT cells from COPD patients when compared to HC and HS subjects as well as in PBT cells from HC stimulated with CSE when compared with unstimulated cells. Tiotropium and Olodaterol alone reduced the increased levels of colocalization between ACh with IL-17A, IL-22 and RORgt in cultured PBT-cells from COPD patients and in PBT-cells from HC subjects stimulated with CSE.

We suggests that cigarette smoke might be able to increase the levels of ACh in PBT cells promoting the switch into PBTh17 cells which produce IL-17A and IL-22 during the systemic inflammation in COPD. The use of anticholinergics as Tiotropium and long-acting β2-agonists as Olodaterol might prevent these events.

Funded by: Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany.

  • Cell biology
  • COPD - mechanism
  • Immunology
  • © 2012 ERS
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Effect of cigarette smoke on peripheral blood Th17 (PBTh17) cells from COPD patients. Role of acetylcholine
Mirella Profita, Giusy Daniela Allbano, Caterina Di Sano, Angela Marina Montalbano, Rosalia Gagliardo, Giulia Anzalone, Loredana Riccobono, Anna Bonanno, Michael Paul Pieper, Mark Gjomarkaj
European Respiratory Journal Sep 2012, 40 (Suppl 56) P1427;

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Effect of cigarette smoke on peripheral blood Th17 (PBTh17) cells from COPD patients. Role of acetylcholine
Mirella Profita, Giusy Daniela Allbano, Caterina Di Sano, Angela Marina Montalbano, Rosalia Gagliardo, Giulia Anzalone, Loredana Riccobono, Anna Bonanno, Michael Paul Pieper, Mark Gjomarkaj
European Respiratory Journal Sep 2012, 40 (Suppl 56) P1427;
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