Abstract
Rationale: Studies have shown that histone deacetylase 9 can affect Tregs function and histone deacetylase inhibitor can inhibit conversion of Tregs into IL-17-producing cells.
Methods: GATA3, IL-4 and HDAC9 mRNA expression level were measured by SYRB Green RT PCR. IL-17 and TGF-β were measured by ELISA. BALB/c mice were randomly divided into three groups, the control group, asthma model group and TSA group. The mice in TSA group were given TSA (1mg/kg) i.p. every other day during sensitization and challenge. The plasma IgE were measured by ELISA, observed the inflammation by HE staining, HDAC9, RORγt and Foxp3 mRNA expression in lung tissue were measured by PCR.
Results: HDAC9 mRNA expression was associated with severity of disease (p<0.01). HDAC9 mRNA expression was correlated with GATA3 mRNA expression positively (p<0.01, r=0.482), the same with IL-4 mRNA expression (p<0.01, r=0.432) and IL-17 (p<0.01, r=0.538), but negatively correlated with TGF-β (p<0.01, r=-0.417). In patients with moderate-severe asthma, HDAC9 mRNA expression was negatively correlated with FEV1% (p<0.01, r=-0.657).
Conclusions: The relationship between expression of histone deacetylase 9 and imbalance of Th17/Treg has been confirmed in asthma. The histone deacetylase inhibitor TSA can control asthma by regulating activity of histone deacetylase.
- © 2012 ERS
