Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

A decreased integrin Mac-1 (CD11b/CD18) induced respiratory burst in neutrophils of COPD patients

Adèle Lo Tam Loi, Corneli van Aalst, Deon Kanters, Laurien Ulfman, Jan-Willem Lammers, Leo Koenderman
European Respiratory Journal 2011 38: p470; DOI:
Adèle Lo Tam Loi
Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Corneli van Aalst
Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Deon Kanters
Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laurien Ulfman
Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jan-Willem Lammers
Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leo Koenderman
Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Introduction: Neutrophils of COPD patients are characterized by changes in activation of the respiratory burst. The aim of this study was to investigate the control of the respiratory burst in neutrophils of COPD patients upon engagement of the integrin Mac-1 (αM/β2; CD11b/CD18).

Methods: A cohort of 34 stable COPD patients (GOLD I-IV) was included in this study. Peripheral blood neutrophils were stimulated with formyl peptides (like fMLF) or anti-Mac-1 antibody (VIM12) and reactive oxygen species (ROS) production was measured by flow cytometry using Dihydrorhodamine 123 (DHR).

Results: The fMLF and anti-Mac-1 antibody (VIM12) induced ROS production in neutrophils from normal controls. Both fMLF and VIM12 were sensitive for priming by platelet-activating factor. The ROS production in neutrophils of COPD patients exhibited a primed phenotype in the context of fMLF. To our surprise the ROS production upon engagement of Mac-1 was markedly reduced in COPD patients compared to normal controls (332.8±64.7 vs. 876.8±275.7 respectively; p < 0.05). This was not associated with a lower expression of Mac-1 on the cell surface measured by flow cytometry.

Conclusion: Our data demonstrated the complex priming phenotype of neutrophils in COPD patients. The unresponsiveness of Mac-1 induced respiratory burst of neutrophils might indicate a shift towards a phenotype more sensitive for bacterial derived molecular patterns.

  • © 2011 ERS
Previous
Back to top
Vol 38 Issue Suppl 55 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
A decreased integrin Mac-1 (CD11b/CD18) induced respiratory burst in neutrophils of COPD patients
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
A decreased integrin Mac-1 (CD11b/CD18) induced respiratory burst in neutrophils of COPD patients
Adèle Lo Tam Loi, Corneli van Aalst, Deon Kanters, Laurien Ulfman, Jan-Willem Lammers, Leo Koenderman
European Respiratory Journal Sep 2011, 38 (Suppl 55) p470;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
A decreased integrin Mac-1 (CD11b/CD18) induced respiratory burst in neutrophils of COPD patients
Adèle Lo Tam Loi, Corneli van Aalst, Deon Kanters, Laurien Ulfman, Jan-Willem Lammers, Leo Koenderman
European Respiratory Journal Sep 2011, 38 (Suppl 55) p470;
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Gene polymorphism of epidermal growth factor receptor (EGFR) and airway hyperresponsiveness (AHR) in young allergic subjects without respiratory symptoms
  • Peanut sensitisation is independently associated with increased airways inflammation in allergic asthma
  • Structure-function relationship between extracellular matrix in airway smooth muscle and dynamics of lung function in asthma
Show more 74. Biomarkers of allergic inflammation

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society