Association of IL-1β (-511 C>T) and IL1RN 86-bp VNTR polymorphism suspectibility with chronic obstrutive pulomary disease between male and female patients in northern Indian population

Abstract

Background: Chronic obstructive pulmonary disease (COPD) is characterized by chronic obstruction of expiratory flow affecting peripheral airways, associated with chronic bronchitis (mucus hypersecretion) and emphysema (destruction of airway parenchyma), together with fibrosis, tissue damage and inflammation of the small airways. Interleukin 1 (IL-1) and other cytokines play a central role in chronic inflammatory diseases. our hypothesis is IL-1β (-511C>T) and IL-1RN 86-bp VNTR polymorphism may be associated with COPD susceptibility.

Methodology: A case–control study was performed in 412 subjects (204 COPD patients and 208 healthy controls) to analyze the association of polymorphisms IL-1β (-511C>T) and IL-1RN 86-bp VNTR of genes in susceptibility to COPD in northern Indian population. All subjects were genotyped through polymerase chain reaction and Restriction fragment length polymorphism.

Results: The frequencies of IL-1β (-511C>T) and IL-1RN (variable number tandem repeat) genotypes, alleles, and haplotypes did not differ significantly between patients and controls. However, In IL1 RN allele 1/2 and 2/2 were significant associated with the female COPD patients, with odds ratio [OR] = 2.60; 95% confidence interval [CI] = 1.24-5.44; p=0.011; OR=5.60; 95% CI = 0.95-32.96; p=0.057).

Conclusion: Both the polymorphism IL-1β (-511C>T) and IL1RN showed sex-specific association with COPD in north India.