Abstract
Objectives: To determine the association of polymorphisms in serine protease inhibitor E2 (SERPINE2), microsomal epoxide hydrolase (EPHX1), transforming growth factor-β1 (TGF-β1) and glutathione S-transferase P1 (GSTP1) with the functional impairment in COPD in Chinese Hans.
Methods: Three hundreds and ten patients with COPD from North China performed pulmonary function test and six-minute walk test. Dyspnea was measured with Medical Research Council (MRC) dyspnea scale, Anxiety and Depression were measured using the Hospital Anxiety and Depression scale (HADS). Forty-two SNPs from the four genes were genotyped using Allele-specific hybridization. Logistic regression and linear regression were used to test for association between these SNPs and the COPD-related traits, assuming dominant (D), recessive (R) and additive (A) genetic model.
Results: Only one SNP (rs3766934) from EPHX1 showed significant association with the six-minute walking distances after Bonferroni correction (A:β=-40.88, P=0.039)One SNP (rs3738040) from EHPX1 showed significant association with anxiety in COPD patients (A: OR=5, P=0.0292). Another SNP (Ile105Val) from GSTP1 showed borderline significance with anxiety symptom (D: OR=2.94, P=0.0545). The haplotype analyses validated the results from the single SNP analyses.
Conclusions: This study provides evidences that genetic variants on EPHX1 and GSTP1, two genes encoding xenobiotic metabolizing enzymes, contribute to the functional impairment of COPD in northern Chinese Hans.
- © 2011 ERS
