Abstract
Fiberglass (FG) is the largest category of man –made mineral fibers (MMVFs). Many tipes of FG are manufactured for specific uses building insulation,air handling,filtration and sound absorbtion. Because of increasing use and potential for widespread human exposurea chronic toxicity instillation study was conducted in Wistar rats, which were found to be sensitive to the induction of mesotheliomas with another MMVF. Four groups of 8 female Wistar rats were included in the study. The animal were devided into three groups per 8 exposed to different doses of FG and one controll group. First group (1-8) was exposed to 6mg dose/0.2ml saline 5days/week 10 weeks; the second (9-16) group was exposed to 10mg/0.2 ml saline 5 days/week 10 weeks, the third group (17-24)was exposed to 12 mg FG/0.2 ml saline solution 5 days/week 10 weeks and the controll group (25-32) was exposed to the same volume of saline. The fibers had been size selected to be rat respirable: length <20μm and diameter ≤1μm. After exposure period of 10 weeks the rats were killed one week after the last exposure. Following preparation of the lungs, they were lavaged with 2x5 ml saline without massage. The lavage fluid was collected in calibrated tubes and harvested volume was recorded. Supernatant was obtained after centrifugation at 1500 r.p.m for 5 minutes and il8 levels were measured. IL 8 levels were ranged between 12.3-20.2pg/ml at the control group, 15.8-40.6 pg/ml first group, 33-86.6pg/ml second group and between 46.5-113.2 pg/ml. Theese findings indicate that il8 levels were dose related and also correlated with lavage citology and histopathological findings.
- © 2011 ERS
