Abstract
Introduction: Determination of VOC present in exhaled breath (EB) may be useful as a noninvasive diagnostic technique in LC.
Objective: To analyze the presence of VOC in the EB in two groups of subject: LC Group and Control Group.
Methods: Descriptive, observational study. LC Group: 29 patients with LC. Control Group: 40 healthy volunteers. (All accepted Informed consent). Breath samples were collected at lung residual functional capacity, with simultaneous sampling of ambient air using BioVOC devices. Analytical technique: Thermal desorption (Markes Int.)-gas chromatography (7890A)-mass spectrometry (5975C-Agilent Tech). VOC analyzed, see table 2. Compounds identified by means of retention time + mass spectrum. Chromatographic column: DB1: 30mx0.25mmx1um (Agilent Tech).
Results:
Conclussions: 1.The presence of VOC (2butanone, Hexanal, Heptanal, Nonanal, Propanoic Acid, nonanoic Acid, Hexane and Tetradecane) exhibits significative differences between groups. 2. Quantification of VOC is extremely difficult because of the great amount of interfering compounds and the low concentrations found. 3.Clinical application of VOC as a noninvasive diagnostic technique in LC is not possible yet due to lack of standardization of methodology.
Acknowledge to Javier Martín from Agilent Tech. for his collaboration.
This work has been financed by FIS PI07/1116; Neumomadrid 2008 and SEPAR 2010.
- © 2011 ERS
