Abstract
Mesenchymal stem cells (MSC) may derive from bone marrow, adipose tissue or lung. Previous studies have shown that bone marrow derived mesenchymal stem cells (BM-MSC) exert beneficial effects in acute lung injury (ALI), but the effects of adipose tissue and lung derived mesenchymal cells (AD-MSC and L-MSC, respectively) have not been evaluated so far. The aim of this study was to investigate the effects of BM-MSC, AD-MSC, L-MSC on lung mechanics and morphometry, as well as inflammation and remodeling in an experimental model of ALI. Forty-eight female Wistar rats (200-250g) received Escherichia coli lipopolysaccharide (LPS) intratracheally (100 μg, ALI) or saline (C). At 48 hours, ALI and C groups were further randomized into subgroups receiving saline (0.05 mL), BM-MSC, AD-MSC, and L-MSC (1×105) intravenously. Bone marrow cells were extracted from four male Wistar rats. The induction of differentiation showed that cells from bone marrow, adipose tissue and lung were able to generate osteocytes, adipocytes and chondrocytes besides presenting CD34-, CD90+ and CD29+ profile. At day 7, mesenchymal cells promoted a reduction in lung static elastance, resistive and viscoelastic pressures, alveolar collapse, collagen fiber content and number of neutrophils in lung tissue, independent of the source. However, the beneficial effects of BM-MSC and AD-MSC on lung parenchyma remodeling were greater than those observed with L-MSC. In conclusion, in the present LPS-induced ALI model, BM-MSC and AD-MSC therapies were more effective than L-MSC at modulating inflammatory and fibrogenic processes.
Supported by: CAPES, PRONEX, FAPERJ, CNPq
- © 2011 ERS
