Figure 1–
CXCL12, CXCR4, and CXCR7 expression and perivascular haematopoietic progenitor cell accumulation in lungs after 3 weeks of hypoxia. a–d) Overexpression of CXCL12 and its two receptors CXCR4 and CXCR7 in total lung protein extracts from hypoxic (H) mice compared with normoxic (N) mice measured by western blotting. *: p<0.05; ***: p<0.001. Expression is normalised towards β-actin. n = 3 in the control group versus n = 5 in the hypoxic group. e–l) Immunohistochemical analysis of vascular localisation of e, f) CXCL12 and their receptors g, h) CXCR4 and i, j) CXCR7, and k, l) accumulation of perivascular c-kit+ and CXCR4+ cells. Normoxia (e, g, i, k) and hypoxia (f ,h, j, l) mouse lung. Scale bars = 50 μm.