Figures
- Figure 1–
1H-nuclear magnetic resonance (NMR) spectra of exhaled breath condensate (EBC) from a healthy subject after a) the standard cleaning protocol recommended by manufacturer’s guidelines and the International Consensus on EBC; b) the cleaning protocol proposed by de Laurentiis et al. 1; c) and d) using a device without reusable condenser parts; and e) 1H-NMR spectra of the condensation of the room air pushed into the system by a continuous air spam device. EBC spectra a), b) and c) were collected from the same healthy subject and spectra c) and d) were collected in the same time scale. f) 1H-NMR spectrum of EBC with similar sample collection and disinfection to 1H-NMR spectrum of EBC published by de Laurentiis et al. 1 (insert; reproduced from 1 with permission from the publisher). f1: first frequency dimension.
- Figure 2–
An ultra-performance liquid chromatography coupled to a time-of-flight mass spectrometer three-dimensional mass matrix of an exhaled breath condensate from a) a healthy subject and b) a subject with chronic obstructive pulmonary disease, acquired using the non-reusable device. m/z: mass to charge.
- Figure 3–
Multivariate analysis using ultra-performance liquid chromatography coupled to a time-of-flight mass spectrometer spectra of exhaled breath condensate from healthy and chronic obstructive pulmonary disease (COPD) subjects. a) Principal components analysis (PCA) score plot shows a separation between groups, which is clearly confirmed by b) partial least squares (PLS) analysis. ○: control; •: COPD. PC1 and 2 are the first two principal components; PLS1 and 2 are the first two partial least squares.
