Figures
Additional Files
Supplementary figures and tables
Files in this Data Supplement:
- Supplementary figure 1 -
Lung inflammatory status of Cftr / mice and effect of ATK. A) PMN counts in BALF of Cftr +/+ and Cftr / mice 24 h and 4 days after LPS challenge. B) Effect of ATK on PMN counts 4 days after LPS challenge - Supplementary figure 2 -
Expression of cPLA2α and inhibition of its activity by ATK. Quantitative representation of western blot analyses of cPLA2α protein (A) and effect of ATK on cPLA2α activity (B) in lung homogenates of Cftr / compared to Cftr +/+ mice - Supplementary figure 3 -
Lung inflammatory status of cPLA2α -/- mice. PMN counts in BALF of cPLA2α +/+ and cPLA2α -/- mice were measured 24 h and 4 days after LPS challenge - Supplementary figure 4 -
Expression of CFTR and cPLA2α in epithelial cells. A) Western blot analyses of cPLA2α in NCI-H292 and Calu-3 cells. B) shows a representative western blot of CFTR in NCI-H292 after incubation with CFTR siRNA, C) shows the quantification of these western blots from 3 independent experiments - Supplementary figure 5 -
Effect of CFTR silencing on MUC5AC and PGE2 secretion by NCI-H292 cells. Incubations were performed as indicated in Figure 7, and then PGE2 (A) and MUC5AC (B) levels were measured in cell cultures - Supplementary table 1 -
Mucus scores in CFTR KO vs WT mice: effect of ATK - Supplementary table 2 -
Clinical characteristics of patients
- Supplementary figure 1 -