Fig. 2— Strain-clustered patients with known epidemiological links. Clustered cases shown as vertical bars on the dendrogram were determined based on identical genotypes as defined by both variable numbers of tandem repeats of mycobacterial interspersed repetitive unit (MIRU-VNTR) typing on 24 genomic loci (MIRU: 2, 4, 10, 16, 20, 23, 24, 26, 27, 31, 39, 40; VNTR: 0424, 0577, 1955, 2163b, 2165, 2347, 2401, 2461, 3171, 3690, 4052, 4156) and spoligotyping. IS6110-restriction fragment length polymorphism patterns were additionally used for identification of strain genetic families. The large cluster corresponding to the S-Brussels strain is boxed. Sex (S), country of birth, age (A) and detection date of tuberculosis patients is given. Types of epidemiological links are represented by the following symbols. •: family link; ♦: social link; ▪: patients living in a single area. P: patient; M: male; F: female.