Fig. 1.—
Effect of intracellular cyclic adenosine monophosphate (cAMP)-increasing agents on eotaxin expression and release. Human airway smooth muscle cells were pretreated with forskolin (10 µM), 8Bromo (8Br)-cAMP (1 mM) or prostaglandin (PG)E2 (1 µM), 30 min later interleukin (IL)‐1β (10 ng·mL−1) was added for 4 h. a) Eotaxin expression, presented as the ratio of eotaxin messenger ribonucleic acid (mRNA) to glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) mRNA. Data are expressed as a percentage of the control response to IL‐1β alone and are represented as mean±sem of four independent experiments, carried out in duplicate using cells cultured from four different donors, cell passages three to six. Duplicate blots from one representative experiment are also shown. b) Eotaxin release after 24 h. *: p< 0.05; **: p<0.01.