Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation
Leader of the Working Group: A. Efthimiadis1, A. Spanevello2, Members of the Working Group:, Q. Hamid, M.M. Kelly, M. Linden, R. Louis, M.M.M. Pizzichini, E. Pizzichini, C. Ronchi, F. Van Overveld, R. Djukanović
European Respiratory Journal 2002 20: 19s-23s; DOI: 10.1183/09031936.02.00001902
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Q. Hamid
M.M. Kelly
M. Linden
R. Louis
M.M.M. Pizzichini
E. Pizzichini
C. Ronchi
F. Van Overveld
Vol 20 Issue 37 suppl
Table of Contents
Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation
Leader of the Working Group: A. Efthimiadis1, A. Spanevello2, Members of the Working Group:, Q. Hamid, M.M. Kelly, M. Linden, R. Louis, M.M.M. Pizzichini, E. Pizzichini, C. Ronchi, F. Van Overveld, R. Djukanović
European Respiratory Journal Jul 2002, 20 (37 suppl) 19s-23s; DOI: 10.1183/09031936.02.00001902
Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation
Leader of the Working Group: A. Efthimiadis1, A. Spanevello2, Members of the Working Group:, Q. Hamid, M.M. Kelly, M. Linden, R. Louis, M.M.M. Pizzichini, E. Pizzichini, C. Ronchi, F. Van Overveld, R. Djukanović
European Respiratory Journal Jul 2002, 20 (37 suppl) 19s-23s; DOI: 10.1183/09031936.02.00001902
Jump To
- Article
- Sputum sample homogenisation
- Duration and temperature of homogenisation
- Volume and concentration of added mucolytic
- Sample filtration
- Total cell count and viability
- Centrifugation and storage of supernatant
- Cytospin centrifugation, staining and counts
- Metachromatic cell staining and counts
- Slide reading quality control
- Immunocytochemical staining
- In situ hybridisation
- Key points
- Outstanding questions
- Appendix: sputum processing methods
- References
- Figures & Data
- Info & Metrics