Fig. 2.—
Immunofluorescence and electron microscopy. a) Confocal laser scanning microscopy with antiserum against Chlamydia spp. shows bright spots within the hypertrophic media of small arteries demonstrating the positive immunofluorescence reaction to Chlamydia spp. (double staining with actin (pale staining), objective magnification ×50). b) Spherical bodies with a diameter of 0.61 µm were seen in lytic areas of the thickened intima, macrophages were found nearby (scanning electron microscopy, original magnification ×10,000). c) These had a double membrane, were lying in small groups, and were sometimes detected within myofibroblasts (transmission electron microscopy, original magnification ×20,000).