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Presence of substance P and neurokinin 1 receptors in human sputum macrophages and U-937 cells

PR Germonpre, GR Bullock, BN Lambrecht, V Van De Velde, WH Luyten, GF Joos, RA Pauwels
European Respiratory Journal 1999 14: 776-782; DOI:
PR Germonpre
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GR Bullock
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BN Lambrecht
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V Van De Velde
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WH Luyten
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GF Joos
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RA Pauwels
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Abstract

Tachykinins such as substance P (SP) may be involved in the pathogenesis of inflammatory airway diseases such as asthma. This study investigated the presence of SP and its receptor in the differentiated macrophage-like U-937 cell line and in macrophages from sputum induced in healthy subjects (n=8). In situ hybridization with digoxigenin-labelled sense and antisense complementary ribonucleic acid (cRNA) probes was used to determine the expression of SP and its receptor (neurokinin (NK)1 receptor). SP-immunoreactive material was detected using a rabbit anti-SP antiserum and the alkaline phosphatase anti-alkaline phosphatase technique. Beta-preprotachykinin (PPT)-I messenger ribonucleic acid (mRNA) encoding SP, was detected using in situ hybridization in differentiated U-937 cells as well as in CD45+ human leukocyte antigen (HLA) DR+ sputum macrophages. The expression of the beta-PPT-I mRNA was increased in lipopolysaccharide (LPS)-stimulated U-937 cells. SP-immunoreactive material was found in differentiated U-937 cells and in CD68+ sputum macrophages. NK1 receptor mRNA was detected in differentiated U-937 cells and sputum macrophages. Incubation of U-937 cells with SP considerably increased the expression of NK1 receptor mRNA. This study demonstrates that human monocytes/macrophages express substance P and that this expression is upregulated by lipopolysacharide. Human monocytes/macrophages also express neurokinin1 receptor messenger ribonucleic acid, suggesting an autocrine effect of substance P on these cells.

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Presence of substance P and neurokinin 1 receptors in human sputum macrophages and U-937 cells
PR Germonpre, GR Bullock, BN Lambrecht, V Van De Velde, WH Luyten, GF Joos, RA Pauwels
European Respiratory Journal Oct 1999, 14 (4) 776-782;

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Presence of substance P and neurokinin 1 receptors in human sputum macrophages and U-937 cells
PR Germonpre, GR Bullock, BN Lambrecht, V Van De Velde, WH Luyten, GF Joos, RA Pauwels
European Respiratory Journal Oct 1999, 14 (4) 776-782;
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