… Dafforn; Surfactant-free purification of membrane proteins with intact native membrane
environment. Biochem Soc Trans 1 June 2011; 39 (3): 813–818. doi: https://doi.org/10.1042/…

One-third of eukaryotic proteins are integrated within membranes, as are the targets of 40%
of approved drugs. However, the lack of a general means of solubilizing, stabilizing and …

Membrane proteins remain a somewhat enigmatic group of biomolecules. On the one hand
they mediate some of the most important processes in biology with molecular mechanisms …

Despite the great importance of membrane proteins, structural and functional studies of these
proteins present major challenges. A significant hurdle is the extraction of the functional …

A major obstacle in the study of membrane proteins is their solubilization in a stable and
active conformation when using detergents. Here, we explored a detergent-free approach to …

… Linear Dichroism and Circular Dichroism: A Textbook on Polarized-Light Spectroscopy
By Bengt Nordén, Alison Rodger and Timothy Dafforn İ B. Nordén, A. Rodger and T. Dafforn, …

The scaffolding protein insulin receptor tyrosine kinase substrate p53 (IRSp53), a ubiquitous
regulator of the actin cytoskeleton, mediates filopodia formation under the control of Rho‐…

Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein
complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent …

It is commonly assumed that a protein must adopt a tertiary structure to achieve its active
native state and that regions of a protein that are devoid of α‐helix or β‐sheet structures are …

… Statistical analysis was carried out using Student's t test (GraphPad Prism), and P<0.05 was
… Robert Ford, Timothy Dafforn and Alice Rothnie analysed the data. Alice Rothnie, Michael …