… These approaches result in much more severe changes in integrin occupancy, F-actin
polymerization, and Rho GTPase activity than are likely to occur physiologically. Additionally, a …

… effect on the intensity of F-actin-specific fluorescence, disrupting F-actin with latrunculin A …
altering the properties of the submembrane F-actin and/or its attachment to the membrane. …

… (F) The average actin fluorescence for cells isolated from stiff and compliant gels decreases
… 3F). Isolated cells tended to have lower average staining intensities than cells in the gel (Fig…

… accurately described by the relationship F=F 0 +2πη eff V t , which is used to evaluate the
membrane surface viscosity η eff and the threshold pulling force F 0 . Effective surface viscosity …

Arterial stiffening is a risk factor for cardiovascular disease, but how arteries stay supple is
unknown. Here, we show that apolipoprotein E (apoE) and apoE-containing high-density …

Cell-derived GPMVs (giant plasma-membrane vesicles) enable investigation of lipid phase
separation in a system with appropriate biological complexity under physiological conditions, …

Many cell types, including neurons, astrocytes and other cells of the central nervous system,
respond to changes in the extracellular matrix or substrate viscoelasticity, and increased …

This study investigates how changes in the level of cellular cholesterol affect inwardly
rectifying K + channels belonging to a family of strong rectifiers (Kir2). In an earlier study we …

… Further, we show that unlike networks of purified F-actin or microtubules, which soften in
compression, vimentin intermediate filament networks stiffen in both compression and extension…

Cell types from many tissues respond to changes in substrate stiffness by actively remodeling
their cytoskeletons to alter spread area or adhesion strength, and in some cases changing …