Characterization of the calmodulin-binding and of the catalytic domains of Bordetella pertussis adenylate cyclase

…, R Sarfati, AM Gilles, R Predeleanu, O Barzu - Journal of Biological …, 1989 - Elsevier
… Arrows indicate the same molecular weight markers (a to d) as in Figs. and 3. … (c); and
soybean trypsin inhibitor, 20,300 (d). AC designates the native adenylate cyclase-CaM complex. …

Binding and endocytosis of heparin by human endothelial cells in culture

T Bârzu, P Molho, G Tobelem, M Petitou… - Biochimica et Biophysica …, 1985 - Elsevier
d = 0.12 #M) and another with lower affinity (K d = 1.37 #M) and a relative large capacity of
binding (1.16.107 molecules/cell) was calculated. The K d … fragments CY 222 (K d = 4_3 ttM) …

Regulation of Expression of the 2-Deoxy-d-Ribose Utilization Regulon, deoQKPX, from Salmonella enterica Serovar Typhimurium

…, T Borza, G Dandanell, AM Gilles, O Barzu… - Journal of …, 2003 - Am Soc Microbiol
Salmonella enterica, in contrast to Escherichia coli K12, can use 2-deoxy-d-ribose as the
sole carbon source. The genetic determinants for this capacity in S. enterica serovar …

Dictyostelium Nucleoside Diphospate Kinase Highly Homologous to Nm23 and Awd Proteins Involved in Mammalian Tumor Metastasis and Drosphila Development

V Wallet, R Mutzel, H Troll, O Barzu… - JNCI: Journal of the …, 1990 - academic.oup.com
… The Gipl7 protein was purified to homogeneity from D. discoideum amoebas and from …
, suggesting that Awd is involved in D. melanogaster development. The high percentage of …

A unique trisaccharide sequence in heparin mediates the early step of antithrombin III activation

M Petitou, T Barzu, JP Herault, JM Herbert - Glycobiology, 1997 - academic.oup.com
… 136, which possess a coil-like conformation in the native state of the protein, would move
to elongate helix-D that already contains Lys 125 and Arg 129. The resulting longer helix …

Characterization of ATP and calmodulin-binding properties of a truncated form of Bacillus anthracis adenylate cyclase

…, S Michelson, AM Gilles, B Laoide, O Barzu - Biochemistry, 1990 - ACS Publications
Revised Manuscript Received December 14, 1989 abstract: The Bacillus anthracis cya gene
encodes a calmodulin-dependent adenylate cyclase. A deletion cya gene product obtained …

Heparin degradation in the endothelial cells

T Barzu, J Van Rijn, M Petitou, G Tobelem, JP Caen - Thrombosis research, 1987 - Elsevier
… I-labeled homogenous heparin ("fraction 13,000 d"), free phenol red to mark the total volume.
Additionally, an octasaccharidk (mw 2,500 d) was used as marker of low molecular weight …

Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement

MB Goldberg, O Barzu, C Parsot… - Journal of …, 1993 - Am Soc Microbiol
… b and d, respectively. Panels: a and b, a moving bacterium within the cytoplasm, trailed by its
actin tail; c and d, … The locations of bacteria are indicated in panels b and d by arrowheads. …

[HTML][HTML] The role of histidine 63 in the catalytic mechanism of Bordetella pertussis adenylate cyclase.

…, E Krin, A Danchin, AM Gilles, P Glaser, O Bârzu - Journal of Biological …, 1992 - Elsevier
… Fluorescence measurements were done with a PerkinElmer LS d B luminescence spectrometer
thermostated at 25 "C using 1 X 1-cm UV-grade quartz cuvettes(sample volume of 2 ml). …

Identification of residues essential for catalysis and binding of calmodulin in Bordetella pertussis adenylate cyclase by site‐directed mutagenesis.

…, E Krin, D Ladant, O Barzu… - The EMBO …, 1989 - embopress.org
In order to identify molecular features of the calmodulin (CaM) activated adenylate cyclase
of Bordetella pertussis, a truncated cya gene was fused after the 459th codon in frame with …