… PCR conditions were as follows: 95C for 10 min, followed by 40 cycles of 95C for 15 s
and 60C for 1 min. Expression of TGF-β relative to the endogenous GAPDH control was …

… Here we demonstrate that infection of BALB/c mice with respiratory syncytial virus (RSV)
induced IL-10 production by CD4 + and CD8 + T cells in the airways at later time points (eg day …

… Real-time PCR was performed using the following conditions: 10 min of denaturation at 95C,
followed by 40 amplification cycles of 15 s at 95C and 60 s at 60C. The relative amounts of …

… ) at D2, D4, D7, and D21 pi (B) Proportion of parental leukocyte populations expressing
ChAT-eGFP in the lungs of naïve ChAT BAC -eGFP mice or animals infected with Nb at D4 pi (C) …

Background Some helminth infections are negatively associated with the prevalence of allergic
disorders, arguing for a modulation of allergic reactions by the parasites, depending on …

… After an incubation for 24 h at 37 C in 5% CO 2 , individual egg batches were washed
extensively in PBS (37 C) to remove virus particles and polybrene. Then, individual egg batches (n…

… How innate host factors, such as C-type lectins, are induced by helminth infection to control
infection or regulate host immunity is not well understood. Some C-type lectins have been …

Respiratory syncytial virus (RSV) causes bronchiolitis in young children and common colds
in adults. There is no licensed vaccine, and prophylactic treatment with palivizumab is very …

Innate immunity is regulated by cholinergic signalling through nicotinic acetylcholine
receptors. We show here that signalling through the M3 muscarinic acetylcholine receptor (M3R) …

… hours at 37C in Stainer-Scholte medium, as described (10); viable counts were determined
by plating on supplemented Bordet-Gengou agar (Difco, Detroit, MI) incubated at 37C for 48 …