Abstract
Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large-scale animal cell cultures.
MeSH terms
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Animals
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Antifoaming Agents / pharmacology*
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Biotechnology / methods
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Cell Death / drug effects
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Cell Death / physiology
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Cells, Cultured
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Culture Media
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Culture Media, Serum-Free
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Hybridomas / cytology*
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Hybridomas / drug effects*
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Mice
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Polyethylene Glycols / pharmacology
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Rats
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Sensitivity and Specificity
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Silicones / pharmacology*
Substances
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Antifoaming Agents
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Culture Media
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Culture Media, Serum-Free
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Silicones
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Polyethylene Glycols