Effect of silicone antifoam on shear sensitivity of hybridoma cells in sparged cultures

Biotechnol Prog. 1993 Sep-Oct;9(5):504-9. doi: 10.1021/bp00023a009.

Abstract

Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large-scale animal cell cultures.

MeSH terms

  • Animals
  • Antifoaming Agents / pharmacology*
  • Biotechnology / methods
  • Cell Death / drug effects
  • Cell Death / physiology
  • Cells, Cultured
  • Culture Media
  • Culture Media, Serum-Free
  • Hybridomas / cytology*
  • Hybridomas / drug effects*
  • Mice
  • Polyethylene Glycols / pharmacology
  • Rats
  • Sensitivity and Specificity
  • Silicones / pharmacology*

Substances

  • Antifoaming Agents
  • Culture Media
  • Culture Media, Serum-Free
  • Silicones
  • Polyethylene Glycols