Abstract
Incubation of isolated rat soleus muscles in the presence of human recombinant TNF-alpha (10,000 U/ml) resulted in an important increase in ubiquitin gene expression (over 50%). Although previous studies involving cytokine administration in vivo (1) have demonstrated an action on ubiquitin-dependent proteolysis, this is the first report demonstrating a direct action of the cytokine on protein breakdown in incubated rat skeletal muscle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cysteine Endopeptidases / biosynthesis*
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Humans
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Multienzyme Complexes / biosynthesis*
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Muscle, Skeletal / drug effects
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Muscle, Skeletal / metabolism*
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Proteasome Endopeptidase Complex
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Proteins / metabolism*
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RNA, Messenger / biosynthesis
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Rats
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Rats, Wistar
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Recombinant Proteins / pharmacology
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Transcription, Genetic / drug effects*
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Tumor Necrosis Factor-alpha / pharmacology*
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Ubiquitins / biosynthesis
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Ubiquitins / metabolism*
Substances
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Multienzyme Complexes
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Proteins
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RNA, Messenger
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Ubiquitins
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Cysteine Endopeptidases
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Proteasome Endopeptidase Complex