Abstract
In order to investigate the epigenetic component of Aiolos regulation, we analyzed the methylation status of its 5' CpG island in relation to histone modifications. Inhibition of CpG methylation restores Aiolos expression, as well as euchromatin-associated markers, in U937 and 1106 mel cell lines. DNA methylation and low levels of euchromatin-associated signatures are observed in U937 and 1106 mel cell lines, while the opposite characterizes Daudi, Jurkat, T and B cells. CpG methylation is not necessary to repress transcription in monocytes and melanocytes where silencing mechanism involves heterochromatin-associated signature. We show that DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetylation
-
Antineoplastic Agents / pharmacology
-
Azacitidine / analogs & derivatives
-
Azacitidine / pharmacology
-
Cell Culture Techniques
-
Cell Line, Tumor
-
Chromatin / metabolism
-
CpG Islands / physiology
-
DNA Methylation
-
Decitabine
-
Drug Combinations
-
Epigenesis, Genetic / physiology*
-
Gene Expression Regulation, Neoplastic*
-
Histone Acetyltransferases / metabolism
-
Histones / metabolism
-
Humans
-
Hydroxamic Acids / pharmacology
-
Ikaros Transcription Factor
-
Jurkat Cells
-
Neoplasms / genetics*
-
Neoplasms / pathology
-
Promoter Regions, Genetic
-
Transcription Factors / genetics*
-
Transcriptional Activation
-
U937 Cells
Substances
-
Antineoplastic Agents
-
Chromatin
-
Drug Combinations
-
Histones
-
Hydroxamic Acids
-
IKZF3 protein, human
-
Transcription Factors
-
Ikaros Transcription Factor
-
trichostatin A
-
Decitabine
-
Histone Acetyltransferases
-
Azacitidine