Cell type-specific involvement of RIG-I in antiviral response

Hiroki Kato; Shintaro Sato; Mitsutoshi Yoneyama; Masahiro Yamamoto; Satoshi Uematsu; Kosuke Matsui; Tohru Tsujimura; Kiyoshi Takeda; Takashi Fujita; Osamu Takeuchi; Shizuo Akira

doi:10.1016/j.immuni.2005.04.010

Cell type-specific involvement of RIG-I in antiviral response

Immunity. 2005 Jul;23(1):19-28. doi: 10.1016/j.immuni.2005.04.010.

Authors

Hiroki Kato¹, Shintaro Sato, Mitsutoshi Yoneyama, Masahiro Yamamoto, Satoshi Uematsu, Kosuke Matsui, Tohru Tsujimura, Kiyoshi Takeda, Takashi Fujita, Osamu Takeuchi, Shizuo Akira

Affiliation

¹ Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.

PMID: 16039576
DOI: 10.1016/j.immuni.2005.04.010

Abstract

Toll-like receptors (TLRs) play an important role in antiviral response by recognizing viral components. Recently, a RNA helicase, RIG-I, was also suggested to recognize viral double-stranded RNA. However, how these molecules contribute to viral recognition in vivo is poorly understood. We show by gene targeting that RIG-I is essential for induction of type I interferons (IFNs) after infection with RNA viruses in fibroblasts and conventional dendritic cells (DCs). RIG-I induces type I IFNs by activating IRF3 via IkappaB kinase-related kinases. In contrast, plasmacytoid DCs, which produce large amounts of IFN-alpha, use the TLR system rather than RIG-I for viral detection. Taken together, RIG-I and the TLR system exert antiviral responses in a cell type-specific manner.

MeSH terms

Animals
DEAD Box Protein 58
DEAD-box RNA Helicases
DNA-Binding Proteins / metabolism
Dendritic Cells / immunology*
Dendritic Cells / metabolism
Dendritic Cells / virology
Fibroblasts / immunology*
Fibroblasts / metabolism
Fibroblasts / virology
Gene Targeting
I-kappa B Kinase
Interferon Regulatory Factor-3
Interferon Type I / metabolism
Interferon-alpha / metabolism
Membrane Glycoproteins / genetics
Membrane Glycoproteins / physiology*
Mice
Newcastle Disease / enzymology
Newcastle Disease / immunology
Newcastle disease virus / immunology
Protein Serine-Threonine Kinases / metabolism
RNA Helicases / genetics
RNA Helicases / physiology*
RNA Virus Infections / enzymology*
RNA Virus Infections / immunology*
Receptors, Cell Surface / genetics
Receptors, Cell Surface / physiology*
Signal Transduction
Toll-Like Receptors
Transcription Factors / metabolism

Substances

DNA-Binding Proteins
Interferon Regulatory Factor-3
Interferon Type I
Interferon-alpha
Irf3 protein, mouse
Membrane Glycoproteins
Receptors, Cell Surface
Toll-Like Receptors
Transcription Factors
Protein Serine-Threonine Kinases
Chuk protein, mouse
I-kappa B Kinase
Ikbkb protein, mouse
Ikbke protein, mouse
Ddx58 protein, mouse
DEAD Box Protein 58
DEAD-box RNA Helicases
RNA Helicases