Abstract
Transforming growth factor (TGF)-beta1 is a multifunctional cytokine that plays important roles in regulating cell growth and differentiation in many biological systems. In this study, we found that gastric tissue-derived Epstein-Barr virus (EBV)-infected epithelial cell lines GT38 and GT39 had resistance to TGF-beta1-mediated growth inhibition and apoptosis compared to a TGF-beta1-susceptible gastric carcinoma cell line HSC-39. However, TGF-beta1 partially induced EBV reactivation in GT38 and GT39 cells, as shown by the induction of EBV immediate-early BZLF1 RNA and its protein product ZEBRA and early antigen-D. The expressions of TGF-beta receptor I and II were detected in GT38 and GT39 cells by Northern and Western blot analyses. Both cell lines spontaneously produced the TGF-beta1, which was sufficient for inhibiting cell growth of HSC-39 cells. Taken together, these data suggest that TGF-beta1 may be a key factor for EBV reactivation and selective growth of EBV-infected epithelial cells in vivo.
Copyright 2001 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activin Receptors, Type I*
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Apoptosis / drug effects*
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Apoptosis / physiology
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Cell Division / drug effects*
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Cell Line
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DNA-Binding Proteins / genetics
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Epithelial Cells
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Flow Cytometry
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Gastric Mucosa
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Gene Expression Regulation / drug effects
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Gene Expression Regulation, Viral / drug effects
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Herpesvirus 4, Human / drug effects*
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / physiology
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Humans
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Protein Serine-Threonine Kinases / genetics
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RNA, Viral / genetics
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Receptor, Transforming Growth Factor-beta Type I
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Receptor, Transforming Growth Factor-beta Type II
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Receptors, Transforming Growth Factor beta / genetics
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Stomach Neoplasms
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Trans-Activators / genetics
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Transcription, Genetic / drug effects*
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Transforming Growth Factor beta / pharmacology*
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Tumor Cells, Cultured
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Viral Proteins / genetics
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Virus Activation / drug effects
Substances
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BZLF1 protein, Herpesvirus 4, Human
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DNA-Binding Proteins
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RNA, Viral
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Receptors, Transforming Growth Factor beta
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Trans-Activators
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Transforming Growth Factor beta
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Viral Proteins
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Protein Serine-Threonine Kinases
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Activin Receptors, Type I
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Receptor, Transforming Growth Factor-beta Type I
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Receptor, Transforming Growth Factor-beta Type II