Chest
Clinical InvestigationsAsthmaThe Relationships Among Hydrogen Peroxide in Expired Breath Condensate, Airway Inflammation, and Asthma Severity
Section snippets
Patients
Fifty nonsmoking stable patients with asthma who were attending the asthma outpatient clinic and 15 healthy, nonatopic, nonsmoking control subjects (Table 1) participated in the study. Thirty-two asthma patients were atopic, as judged by positive responses to skin prick tests of six common allergens and the elevated serum level of IgE. A diagnosis of asthma was established according to the National Heart, Lung, and Blood Institute (NHLBI) guidelines for the diagnosis and management of asthma.1
H2O2 Concentration in Expired Breath Condensate, Airway Inflammatory Cells, and ECP in Induced Sputum
Repeated measurements on 2 consecutive days showed a mean within-subject difference of 0.09 μM (SD, 0.03 μM) for patients and 0.07 μM (SD, 0.04 μM) for healthy subjects. The stability of the H2O2 in frozen samples showed no significant differences among the four measurements (after 2 days: mean, 0.54 μM [SD, 0.21 μM]; after 1 week: mean, 0.58 μM[SD, 0.3 μM]; after 2 weeks: mean, 0.6 μM [SD, 0.3 μM]; after 3 weeks: mean, 0.57 μM [SD, 0.4 μM]; p = 0.43).
The mean H2O2 concentration in expired
Discussion
In a cross-sectional study from a wide spectrum of patients with asthma of stable severity, we have demonstrated for the first time that the increased oxidative stress, as assessed by H2O2 levels in expired breath condensate, is related to eosinophilic inflammation, while in more severe forms of the disease neutrophils might participate to a lesser extent. This increase in H2O2 levels is affected by both the severity of the disease and the use of ICS. We interpret these findings as a
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