Chest
Clinical Investigations: Smoking and Nitric Oxide: ArticlesRole of Endogenous Nitric Oxide in Airflow Obstruction in Smokers
Section snippets
Materials and Methods
The experiments were approved by the human ethics committee of our university, and informed consent for participation was obtained from each subject. Nineteen healthy volunteers (all male) were allocated to two groups (Table 1): nonsmoking (n=9, mean [SE] 32.3 [3.4] years) and smoking (n=10,35.1 [1.7] years) subjects. Subjects with an asthmatic component, hypertension, or respiratory infection within the 2 weeks preceding the study were excluded. Smokers were asked not to smoke during the 2-h
Results
The two groups were age matched, and in both, pulmonary function was within the normal range, but FEV1.0% and maximum expiratory flow at 25% and 50% vital capacity were significantly lower in smokers than in nonsmokers. In nonsmokers, the concentration of exhaled NO increased after breath-holding in a time-dependent manner (Fig 1). However, the concentration of exhaled NO was not affected by breathholding in smokers. The level of exhaled NO was higher in nonsmokers than in smokers, and this
Discussion
In this study, we examined healthy male volunteers who were relatively young and had normal pulmonary function to evaluate the effect of smoking alone. We used the difference in NO concentration (ΔNO) as a parameter to determine the degree of airflow obstruction because it is a sensitive indicator of NO synthesis in the respiratory tract. In nonsmokers, the increase in NO in exhaled air after breath-holding was probably due to accumulation of basally excreted NO, since the amount of NO in
References (16)
- et al.
Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans
Biochem Biophy Res Commun
(1991) - et al.
Single-breath nitric oxide measurements in asthmatic patients and smokers
Lancet
(1994) - et al.
Nitric oxide: physiology, pathophysiology and pharmacology
Pharmacol Rev
(1991) Nitric oxide as a secretory product of mammalian cells
FASEB J
(1992)- et al.
Endogenous nitric oxide as a probable modulator of pulmonary circulation and hypoxic pressor response in vivo.
Acta Physiol Scand
(1990) - et al.
Inhaled nitric oxide for the adult respiratory distress syndrome
N Engl J Med
(1993) - et al.
Bronchodilator action of nitric oxide in guinea pigs
J Clin Invest
(1992) - et al.
Endogenous nitric oxide in single exhalations and the change during exercise
Am Rev Respir Dis
(1993)
Cited by (20)
Lycopene mitigates pulmonary emphysema induced by cigarette smoke in a murine model
2019, Journal of Nutritional BiochemistryCitation Excerpt :One minute after cessation of smoking, levels of expired nitric oxide increase in smokers. Two hours after smoking, however, levels of expired nitric oxide are lower in smokers than nonsmokers [1,63]. This sustained reduction in expired nitric oxide in smokers may result from superoxide and peroxynitrite-dependent inflammatory and conversion processes [1].
Measurement of exhaled nitric oxide in humans and animals
1999, Pulmonary Pharmacology and TherapeuticsModulation of lung function by increased nitric oxide production
2017, Journal of Clinical and Diagnostic ResearchA study of fraction of exhaled nitric oxide levels as a diagnostic marker in patients with bronchial asthma
2013, Journal, Indian Academy of Clinical Medicine