Chest
Original ResearchCOPDMicroRNA-199a-5p Is Associated With Hypoxia-Inducible Factor-1α Expression in Lungs From Patients With COPD
Section snippets
Lung Tissue Samples
Lung tissue samples from 55 patients were included in this study. We divided the patients into four groups according to the GOLD (Global Initiative for Chronic Obstructive Lung Disease) classification. Ten samples were from patients with normal lung function (no COPD); the remainder of the samples were from patients with mild (n = 13; stage I, GOLD classification), moderate (n = 17; stage II, GOLD classification), and severe (n = 15; stage III and IV, GOLD classification) COPD. Table 1
Patient Characteristics
The data and characteristics describing the patients from which the lung tissue samples (LTRC) have been derived are displayed in Table 1. The lung samples from never smokers with normal lung function were all from female patients. Although a histopathologic diagnosis of lung cancer had been made in 24 of 55 patients, the tissue samples investigated were all histologically cancer-free and represented tissue highly remote from any tumor. The LTRC data set did not provide blood gas information
Discussion
The destruction of lung tissue in patients with COPD and emphysema has been well characterized by histologic studies and inflammatory and per se noninflammatory pathomechanisms have been proposed and discussed,24 also in the context of a homeostatic adult lung structure maintenance program.25 We have reported a decreased expression of HIF-1α protein in lungs from patients with COPD and varying degrees of lung function impairment.14 Here, to our knowledge, we report for the first time the
Acknowledgments
Author contributions: Dr Mizuno: contributed to designing and organizing the experiments, carrying out the data analysis, and writing of the manuscript.
Dr Bogaard: contributed to the design of the study and revising the manuscript.
Dr Gomez-Arroyo: contributed to laboratory measurements, data analysis, and writing of the manuscript.
Dr Alhussaini: contributed to laboratory measurements, data analysis, and writing of the manuscript.
Dr Kraskauskas: contributed to laboratory measurements, data
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Funding/Support: This study was supported by the National Institutes of Health [Grant N01-HR-46160-3], and funds from the Victoria Johnson Center for Lung Research of the Virginia Commonwealth University.
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