Asthma, Rhinitis, Other Respiratory DiseasesHealthy subjects express differences in clinical responses to inhaled lipopolysaccharide that are related with inflammation and with atopy☆,☆☆
Section snippets
Subjects
A group of 15 healthy, asymptomatic, nonsmoking subjects, preselected for their ability to produce an adequate sample of induced sputum, were included. The mean age was 31.1 (±3.0) years; 8 were female and 7 male. Total and specific blood IgE levels (CAP system; Pharmacia, Uppsala, Sweden) for airborne allergens were determined. On the basis of positive (≥4-mm wheal) skin prick test results for the most frequent allergens (Bencard, Worthing, UK) and/or positive specific IgE for at least 1
Results
The time-related response to 50 μg LPS was analyzed to define the time at which body temperature, lung function, and blood inflammatory indicators were at maximum (Fig 1).
Discussion
We have shown that healthy subjects exposed to LPS express differences in changes in body temperature, AR, and FEV1. Subjects who had an increase in temperature showed an increase in indicators of inflammation in the blood (systemic response). Those who had an increase in AR showed an increase in indicators of airway inflammation (local response) but not in the blood. Those who had a decrease in FEV1 did not show an increase in inflammatory markers. The atopic status was inversely related to
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2015, ChestCitation Excerpt :Opposing effects of endotoxin were seen in atopic vs nonatopic children, with a trend toward decreased wheeze in atopic children but a significant increase in wheeze in nonatopic children from nonfarming households. Bronchial challenge studies in healthy subjects further demonstrate that atopy modifies the human response to endotoxin, with atopic subjects showing significantly decreased systemic inflammatory responses to inhaled endotoxin compared with nonatopic subjects.26 It may seem puzzling that classroom-specific airborne endotoxin levels were associated with asthma morbidity whereas settled dust levels were not.
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Supported by grant BMH-CT 96-0105 from EC work program Biomed and Health. Dr Corazza was partially supported by a grant from the Medical Council of Brugmann University Hospital (ULB, Brussels).
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Reprint requests: O. Michel, MD, Clinic of Allergology and Respiratory Diseases, Centre Hospitalier Universitaire Saint-Pierre, Université Libre de Bruxelles, Rue Haute 290, B 1000 Brussels, Belgium.