Clinical-alimentary tractPeripheral and Intestinal Regulatory CD4+CD25high T Cells in Inflammatory Bowel Disease
Section snippets
Subjects
Peripheral blood from 46 patients with CD (24 patients with active and 22 patients with inactive disease; 22 women, 24 men; mean age, 42 years; range, 18–74 years), 28 patients with UC (14 patients with active and 14 patients with inactive disease; 13 women, 15 men; mean age, 41 years; range, 25–89 years), 11 patients with acute diverticulitis (7 women, 4 men; mean age, 59 years; range, 45–73 years), and 16 healthy volunteers (9 women, 7 men; mean age, 31 years; range, 23–57 years) were
Peripheral Blood Treg Are Increased in Inactive and Decreased in Active IBD
Similar to published data,14, 18, 22 we found that CD4+CD25high Treg cells are present in peripheral blood lymphocytes of healthy individuals at a mean of 1.64% (range, 0.6%–3.17%). On flow cytometry, these human Treg cells appear as a subpopulation to the right from the major population of CD4+CD25− and CD4+CD25low cells (Figure 1A).
To determine whether changes in frequency of CD25high Treg cells are present in IBD and correlate with disease activity, we analyzed CD25high surface expression in
Discussion
CD4+CD25+ Treg cells have potent antiinflammatory capacity in animal models of intestinal inflammation.29 To the best of our knowledge, this is the first study that specifically compares the frequency of CD4+CD25+ Treg cells in active vs inactive IBD.
On the mucosal level, we find that the frequency of CD4+CD25+ Treg cells determined by the number of CD4+CD25high T cells, FOXP3+ T cells, and FOXP3 transcripts are consistently increased in active IBD lesions compared with noninflamed areas.
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Supported by Deutsche Forschungsgemeinschaft grant A2SFB633 (to R.D.) and Z1SFB633 (to M.Z.) and by a grant from the German Federal Department for Research and Education “Kompetenznetz chronisch entzündliche Darmerkrankungen.”