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Distinct lineages of TH1 cells have differential capacities for memory cell generation in vivo

Nature Immunology volume 3, pages 852–858 (2002)Cite this article

Abstract

We studied here the long-term maintenance of distinct populations of T helper type 1 (TH1)-lineage cells in vivo and found that effector TH1 cells, defined by their secretion of interferon-γ (IFN-γ), are short-lived and do not efficiently develop into long-term memory TH1 cells. In contrast, a population of activated TH1-lineage cells that did not secrete IFN-γ after primary antigenic stimulation persisted for several months in vivo and developed the capacity to secrete IFN-γ upon subsequent stimulation. These data suggest that a linear differentiation pathway, as defined by the transition from IFN-γ–producing to resting memory cells, is relatively limited in vivo and support a revised model for TH1 memory differentiation.

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Figure 1: Survival of TH 1 effector cells in vivo after adoptive transfer.
Figure 2: Characterization and isolation of IFN-γ+ and IFN-γ− cells from TH1 week 1 cultures.
Figure 3: The fate of IFN-γ+ and IFN-γ− cells generated in vivo after adoptive transfer.

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Acknowledgements

We thank B. Marshal for editorial assistance and X. Tai for technical help with cell preparation.

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Authors and Affiliations

  1. Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20892-3005, MD, USA

    Chang-you Wu, Joanna R. Kirman, Masashi J. Rotte, Dylan F. Davey, Elizabeth G. Rhee, Brenda L. Freidag & Robert A. Seder

  2. Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20892-3005, MD, USA

    Steve P. Perfetto

  3. Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20892-3005, MD, USA

    Brenna J. Hill & Daniel C. Douek

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  1. Chang-you Wu
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Correspondence to Robert A. Seder.

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Wu, Cy., Kirman, J., Rotte, M. et al. Distinct lineages of TH1 cells have differential capacities for memory cell generation in vivo. Nat Immunol 3, 852–858 (2002). https://doi.org/10.1038/ni832

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