Abstract
We examined the effects of lansoprazole, aproton-pump inhibitor, on peripheral blood mononuclearcells in healthy subjects in comparison with ranitidine.Ten healthy volunteers were randomly divided into two groups and given either lansoprazole (30 mgdaily for 2 days) or ranitidine (150 mg daily for 21days). Peripheral blood was collected before and 7, 14,and 21 days after the start of treatment. Mononuclear cells were isolated by densitometriccentrifugation and were examined for adhesion molecules(ICAM-1, VLA4, SLex), membrane markers of themonocyte/macrophage series, and lymphocyte phenotypes.The number of cells expressing adhesion molecules, thenumber of monocytes/macrophages, and lymphocytephenotypes were the same in Helicobacter pylori-positiveand-negative subjects. The number of cells expressing ICAM-1 was significantly decreased seven daysafter the start of lansoprazole treatment, and thischange persisted until day 14, while ranitidine had noeffect. The number of monocytes (identified by Leu-M3 positivity) was decreased seven days after thestart of treatment in both groups, but predominantly inthe lansoprazole group. No other changes were observedon administration of either drug. These results suggest that short-term treatment withlansoprazole causes persistent inhibition ofinflammatory responses irrespective of the presence ofH. pylori infection. This effect may indicate a possiblenew mechanism of action of proton-pump inhibitors other thaninhibition of acid secretion.
Article PDF
Similar content being viewed by others
REFERENCES
Labenz J, Blum AL, Bayerdorffer E, Meining A, Stolte M, Boresch G: Curing Helicobacter pylori infection in patients with duodenal ulcer may provoke reflux esophagitis. Gastroenterology 112:1442–1447, 1997
Baak LC, Jansen JB, Biemond I, Lamers CB: Weekend treatment with 20 and 40 mg omeprazole: effect on intragastric pH, fasting and postprandial serum gastrin, and serum pepsinogens. Gut 32:977–982, 1991
Bianchi-Porro G, Corinaldesi R, Lazzaroni M, Barbara L, Capurso L, Paoluzi P, Mangiameli A, Sabbatini F, Franceschi M, Bolling E: Long term treatment with omeprazole 20 mg three days a week or 10 mg daily in the prevention of duodenal ulcer relapse. Aliment Pharmacol Ther 8:541–548, 1994
Hoshino E, Umeda N, Sano J, Miki K, Yahagi N, Oka M, Kimura M, Miyashita H: Lansoprazole for maintenance therapy of peptic ulcer disease: Weekend full-dose or everyday half-dose administration? J Clin Gastroenterol 20(suppl2):S72-S74, 1995
Catalano F, Liberti A, Branciforte G, Catanzaro R, Bucceri AM, Blasi A: Twelve-month omeprazole vs ranitidine in the treatment of Helicobacter pylori positive patients with healed duodenal ulcer. Ital J Gastroenterol 27:21–25, 1995
Interdisciplinary Group for Ulcer Study: Six month of omeprazole 20 mg daily, 20 mg every other day or 40 mg at weekends in duodenal ulcer patients: A multicenter, prospective, comparative study. Digestion 56:181–186, 1995
Savarino V, Mera GS, Zentilin P, Cutela P, Mele MR, Perilli D, Vassallo A, Zambotti A, Mansi C, Celle G: Antisecretory effects of three omeprazole regimens for maintenance treatment in duodenal ulcer. Dig Dis Sci 39:1473–1482, 1994
Suzuki M, Nakamura M, Mori M, Miura S, Tsuchiya M, Ishii H: Lansoprazole inhibits oxygen-derived free radical production from neutrophils activated by Helicobacter pylori. J Clin Gastroenterol 20(suppl2):S93-S96, 1997
Suzuki M, Mori M, Miura S, Suematsu M, Fukumura D, Kimura H, Ishii H: Omeprazole attenuates oxyge n-derived free radical production from human neutrophils. Free Radic Biol Med 21:727–731, 1996
Watanabe T, Arakawa T, Fukuda T, Higuchi K, Kobayashi K: Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1ß . Am J Pathol 150:971–979, 1997
Sugawara S, Abo T, Kumagai K: A simple method to eliminate the antigenicity of surface class I MHC molecules from the membrane of viable cells by acid treatment at pH 3. J Immunol Methods 100:83–90, 1987
Satoh M, Seki S, Hashimoto W, Ogasawara K, Kobayashi T, Kumagai K, Matsuno S, Takeda K: Cytotoxic γδ or αβ T cells with a natural killer cell maker, CD56, induced from human peripheral blood lymphocytes by a combination of IL-12 and IL-2. J Immunol 157:3886–3892, 1996
Crabtree JE, Shallcross TM, Heatley RV, Wyatt JI: Mucosal tumour necrosis factor a and interleukin-6 in patients with Helicobacter pylori associated gastritis. Gut 32:1473–1477, 1991
Crabtree JE, Peichl P, Wyatt JI, Stachl U, Lindley IJD: Gastric interleukin-8 and IgA IL-8 autoantibodies in Helicobacter pylori infection. Scand J Immunol 37:65–70, 1993
Crabtree JE, Wyatt JI, Trejdosiewicz LK, Peichl P, Nichols PH, Ramsay N, Primrose JN, Lindley IJD: Interleukin-8 expre ssion in Helicobacter pylori infected, normal, and neoplastic gastroduodenal mucosa. J Clin Pathol 47:61–66, 1994
Fan X-G, Chua A, Fan X-J, Keeling PWN: Increased gastric production of interleukin-8 and tumour necrosis factor in patients with Helicobacter pylori infection. J Clin Pathol 48:133–136, 1995
Moss SF, Legon S, Davies J, Calam J: Cytokine gene expression in Helicobacter pylori associated antral gastritis. Gut 35:1567–1570, 1994
Noach LA, Bosma NB, Jansen J, Hoek FJ, van Deventer SJH, Tytgat GNJ: Mucosal tumor necrosis factor-alpha, interleukin-1β, and interleukin-8 production in patients with Helicobacter pylori infection. Scand J Gastroenterol 29:425–429, 1994
Santucci L, Fiorucci S, Di Matteo FM, Morelli A: Role of tumor necrosis factor alpha release and leukocyte margination in indomethacin-induced gastric injury in rats. Gastroenterology 108:393–401, 1995
Santucci L, Fiorucci S, Brunori PM, Di Matteo FM, Chiorean M, Morelli A: Tumor necrosis factor alpha receptor antagonists protect against indomethacin-induced gastric mucosal injury in rats. Gastroenterology 108:A209, 1995
Cho CH, Mei Q, Zhou YL: The role of interleukin-8 in the development of stress-induced gastric ulceration. Gastroenterology 110:A81, 1996
Shintani F, Nakaki T, Kanba S, Kato R, Asai M: Role of interleukin-1 in stress response. A putative neurotransmitter. Mol Neurobiol 10:47–71, 1995
Rights and permissions
About this article
Cite this article
Ohara, T., Arakawa, T. Lansoprazole Decreases Peripheral Blood Monocytes and Intercellular Adhesion Molecule-1-Positive Mononuclear Cells. Dig Dis Sci 44, 1710–1715 (1999). https://doi.org/10.1023/A:1026604203237
Issue Date:
DOI: https://doi.org/10.1023/A:1026604203237