Asthma and lower airway diseaseLevels of nitric oxide oxidation products are increased in the epithelial lining fluid of children with persistent asthma
Section snippets
Sample
Children 5 to 17 years of age with symptomatic asthma attending an asthma clinic at Emory University were invited to participate in this study. Asthmatic children met published criteria for persistent asthma14 and had a history of at least a 12% change in FEV1 after albuterol administration.15 Severe asthma was diagnosed according to criteria developed by the National Institutes of Health/National Heart, Lung, and Blood Institute Severe Asthma Research Program,1 which were adapted from the
Results
Initially, 49 asthmatic children (severe asthma, n = 32), 7 pediatric control subjects, and 20 healthy adult control subjects were recruited for this study. However, 6 children, including 2 pediatric control subjects, 2 subjects with mild-to-moderate asthma, and 2 subjects with severe asthma, were infected with Streptococcus pneumoniae, Haemophilus influenzae, and/or Moraxella catarrhalis and were excluded from data analysis because of potential denitrification.23 The features of the excluded
Discussion
To our knowledge, this is the first study to directly measure NO oxidation products in the ELF of children with persistent asthma. Compared with control subjects, children with mild-to-moderate and severe allergic asthma had increased concentrations of nitrite, nitrate, and nitrotyrosine in the ELF that were consistently higher in the proximal versus the distal airways. Contrary to our hypothesis, we did not detect significant differences in concentrations of NO oxidation products between
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Cited by (0)
Supported with funds from National Institutes of Health/National Institute of Nursing Research KO1 NR010548, National Institutes of Health/National Center for Research Resources K12 RR017643, and National Institutes of Health/National Heart, Lung, and Blood Institute Severe Asthma Research Program RO1 HL69170.
Disclosure of potential conflict of interest: W. G. Teague is on the speakers' bureau for Merck and Co; receives research support from the National Institutes of Health/National Heart, Lung, and Blood Institute, the American Lung Association, and the Centers for Disease Control and Prevention; is on the review panel for the American Thoracic Society; and is on the advisory board for WESTAT–Children's Health Study. The rest of the authors have declared that they have no conflict of interest.
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See the acknowledgments for a complete listing of the Severe Asthma Research Program contributors.