Abstract
Background and Aim
With the development of immunology, the role of immune inflammation in idiopathic pulmonary arterial hypertension (IPAH) has attracted interest. Recently, it was discovered that dendritic cells, which are key players in immune inflammation, are implicated in the pathogenesis of IPAH. To elucidate the role of dendritic cells in human IPAH, we compared the changes in the number and immunological function of monocyte-derived dendritic cells (MoDCs) from the peripheral blood of patients with IPAH and healthy controls.
Methods
The numbers of MoDC subsets (including plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells (mDCs)) in circulating peripheral blood mononuclear cells (PBMCs) was analyzed by flow cytometry, and the concentrations of interleukin (IL)-12, IL-10, and tumor necrosis factor-alpha were measured by enzyme-linked immunosorbent serologic assay kits. The morphology, phenotypic expression, and the ability to stimulate T cell proliferation of MoDCs, cultured from PBMCs in vitro with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4, was analyzed by microscopy, flow cytometry, and MTT assay.
Results
The results of the study are as follows: (1) The number of circulating mDCs was lower in IPAH patients than in controls (0.07 ± 0.01% to 0.14 ± 0.02%; p < 0.05). (2) IL-12 levels were higher in IPAH patients than in controls (p < 0.05). (3) MoDCs showed higher expression of CD1a (53.34 ± 7.43% to 19.29 ± 7.37%; p < 0.05), and lower expression of costimulatory molecule CD86 (64.54 ± 5.93% to 87.04 ± 4.82%; p < 0.05), and less ability to simulate T cell proliferation (when the ratio is 1:10) compared to the controls.
Conclusions
The study shows that it is possible to obtain typical DCs by culturing PBMCs from patients with IPAH with GM-CSF and IL-4, and it demonstrates that patients with IPAH have a significant change in the number of mDC and a marked immune deficiency of MoDCs.
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References
Dorfmuller P, et al. Inflammation in pulmonary arterial hypertension. Eur Respir J. 2003;22(2):358–63.
Nicolls MR, et al. Autoimmunity and pulmonary hypertension: a perspective. Eur Respir J. 2005;26(6):1110–8.
Ogawa A, et al. Prednisolone inhibits proliferation of cultured pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension. Circulation. 2005;112(12):1806–12.
Bellotto F, et al. Effective immunosuppressive therapy in a patient with primary pulmonary hypertension. Thorax. 1999;54(4):372–4.
Sanchez O, et al. Immunosuppressive therapy in connective tissue diseases-associated pulmonary arterial hypertension. Chest. 2006;130(1):182–9.
Perros F, et al. Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension. Eur Respir J. 2007;29(3):462–8.
Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature. 1998;392(6673):245–52.
Fehervari Z, Sakaguchi S. CD4+ Tregs and immune control. J Clin Invest. 2004;114(9):1209–17.
Shortman K, Liu YJ. Mouse and human dendritic cell subtypes. Nat Rev Immunol. 2002;2(3):151–61.
Pietra GG, et al. Pathologic assessment of vasculopathies in pulmonary hypertension. J Am Coll Cardiol. 2004;43(12_Suppl_S):25S–32.
Fartoukh M, et al. Chemokine macrophage inflammatory protein-1alpha mRNA expression in lung biopsy specimens of primary pulmonary hypertension. Chest. 1998;114(1):50S–51S.
Balabanian K, et al. CX3C chemokine fractalkine in pulmonary arterial hypertension. Am J Respir Crit Care Med. 2002;165(10):1419–25.
Gugl A, et al. Two polymorphisms in the fractalkine receptor CX3CR1 are not associated with peripheral arterial disease. Atherosclerosis. 2003;166(2):339–43.
Odobasic D, et al. CD80 and CD86 costimulatory molecules regulate crescentic glomerulonephritis by different mechanisms. Kidney Int. 2005;68(2):584–94.
Pentcheva-Hoang T, et al. B7-1 and B7-2 selectively recruit CTLA-4 and CD28 to the immunological synapse. Immunity. 2004;21(3):401–13.
Porcelli SA, et al. The CD1 family of lipid antigen-presenting molecules. Immunol Today. 1998;19(8):362–8.
Karmochkine M, et al. High prevalence of antiphospholipid antibodies in precapillary pulmonary hypertension. J Rheumatol. 1996;23(2):286–90.
Negi VS, et al. Antiendothelial cell antibodies in scleroderma correlate with severe digital ischemia and pulmonary arterial hypertension. J Rheumatol. 1998;25(3):462–6.
Tamby MC, et al. Antibodies to fibroblasts in idiopathic and scleroderma-associated pulmonary hypertension. Eur Respir J. 2006;28(4):799–807.
Rich SD, Ayres SM, et al. Primary pulmonary hypertension:a national prospective study. Ann Intern Med. 1987;107:216–23.
Penna G, et al. Differential migration behavior and chemokine production by myeloid and plasmacytoid dendritic cells. Hum Immunol. 2002;63(12):1164–71.
Gill MA, et al. Blood dendritic cells and DC-poietins in systemic lupus erythematosus. Hum Immunol. 2002;63(12):1172–80.
Pacanowski J, et al. Reduced blood CD123+ (lymphoid) and CD11c + (myeloid) dendritic cell numbers in primary HIV-1 infection. Blood. 2001;98(10):3016–21.
Duan X-Z, et al. Decreased numbers and impaired function of circulating dendritic cell subsets in patients with chronic hepatitis B infection (R2). J Gastroenterol Hepatol. 2005;20(2):234–42.
Vuckovic S, et al. Decreased blood dendritic cell counts in type 1 diabetic children. Clin Immunol. 2007;123(3):281–8.
Yilmaz A, et al. Decrease in circulating myeloid dendritic cell precursors in coronary artery disease. J Am Coll Cardiol. 2006;48(1):70–80.
Lotze MT, Deisseroth A, Rubartelli A. Damage associated molecular pattern molecules. Clin Immunol. 2007;124(1):1–4.
Rubartelli A, Lotze MT. Inside, outside, upside down: damage-associated molecular-pattern molecules (DAMPs) and redox. Trends Immunol. 2007;28(10):429–36.
Bowers R, et al. Oxidative stress in severe pulmonary hypertension. Am J Respir Crit Care Med. 2004;169(6):764–9.
Wang X-X, et al. Transplantation of autologous endothelial progenitor cells may be beneficial in patients with idiopathic pulmonary arterial hypertension: a pilot randomized controlled trial. J Am Coll Cardiol. 2007;49(14):1566–71.
Duan XZ, Zhuang H, Wang M, Li HW, Liu JC, Wang FS. Decreased numbers and impaired function of circulating dendritic cell subsets in patients with chronic hepatitis B infection (R2). J Gastroenterol Hepatol 2005;20:234–42.
Ulsenheimer A, Gerlach JT, Jung MC, et al. Plasmacytoid dendritic cells in acute and chronic hepatitis C virus infection. Hepatology 2005;41:643–51.
Uehira K, Amakawa R, Ito T, et al. Dendritic cells are decreased in blood and accumulated in granuloma in tuberculosis. Clin Immunol 2002;105:296–303.
Acknowledgements
The authors would like to thank Professor Junzhu Chen for advice and constructive discussion. We are also grateful to the patients for participating in the study.
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This work was supported by the project of the Natural Science Foundation of China (306708886/C03030201), Beijing; Science and Technology project of Zhejiang Province (2008C23044), and Medical Science and Technology project of Zhejiang Province (2006A048, 2007B054), Hangzhou.
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Wang, W., Yan, H., Zhu, W. et al. Impairment of Monocyte-derived Dendritic Cells in Idiopathic Pulmonary Arterial Hypertension. J Clin Immunol 29, 705–713 (2009). https://doi.org/10.1007/s10875-009-9322-8
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DOI: https://doi.org/10.1007/s10875-009-9322-8