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CC16 Inhibits the Migration of Eosinophils Towards the Formyl Peptide fMLF but not Towards PGD2

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Abstract

Clara cell 16-kDa (CC16) is an anti-inflammatory protein chiefly produced in the lung epithelium. CC16 has been shown to inhibit the migration of rabbit neutrophils and human monocytes toward the formyl peptide N-formyl-methionine-leucin-phenylalanin (fMLF). Eosinophils migrate towards prostaglandin D2 (PGD2) and CC16 has been shown to bind to PGD2. Therefore we investigated if CC16 could inhibit the migration of human eosinophils and neutrophils towards fMLF and/or PGD2. Migration of eosinophils and neutrophils was assessed in a microplate migration system using specific ligands and receptor antagonists. CC16 inhibited the migration of eosinophils and neutrophils toward fMLF, which is likely to result from the interaction of CC16 with members of the formyl-peptide receptor family. However, CC16 did not inhibit eosinophil migration towards PGD2. We therefore propose that CC16 may down-modulate the entry of human eosinophils and neutrophils into the airways during inflammation in the lung.

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Acknowledgement

We thank the healthy volunteers for taking part in the study. We also thank Aprile L Pilon at Claragen Inc for providing recombinant human CC16. This study was supported by the Swedish Research Council (grant K2002-745X-14455-01A), by the Swedish Foundation for Health Care Sciences and Allergy Research, by Cancer-och Allergifonden, by the Swedish Asthma and Allergy Association, by the Sahlgrenska Academy, University of Gothenburg and by Frimurare Barnhusdirektionen.

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Authors and Affiliations

  1. Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10A, 413 46, Göteborg, Sweden

    Sofi Johansson, Kerstin Andersson & Anna Rudin

  2. Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden

    Sofi Johansson & Göran Wennergren

  3. Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden

    Kerstin Andersson & Christine Wennerås

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  1. Sofi Johansson
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  2. Kerstin Andersson
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  4. Christine Wennerås
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  5. Anna Rudin
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Correspondence to Sofi Johansson.

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Johansson, S., Andersson, K., Wennergren, G. et al. CC16 Inhibits the Migration of Eosinophils Towards the Formyl Peptide fMLF but not Towards PGD2 . Inflammation 32, 65–69 (2009). https://doi.org/10.1007/s10753-008-9103-1

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