Abstract
Alveolar macrophages (AMs) were recruited by bronchoalveolar lavage (BAL) from human smokers before and one, three, and six months after smoking cessation. The metabolic activity of the AM was quantified as luminol-enhanced chemiluminescence (CL) both at rest and after in vitro stimulation with phorbol myristate acetate (PMA). The resting CL values did not differ before and after smoking cessation. The activity after PMA stimulation was unaltered at one and three months. However, the maximal metabolic response, as well as the rate, were significantly (P < 0.02 andP < 0.01, respectively) higher at six months, compared to prior smoking cessation. In addition, the time to reach the maximal peak was reduced after six smoke-free months, indicating a more rapid cell activation. The cell concentration in the BAL-fluid decreased (P < 0.001) as soon as after one smoke-free month and remained low at the following lavages. The lower metabolic response one and three months after smoking cessation, and the increased response six months after, together with a rapid normalization of the cell concentration in the BAL fluid, may be explained by the persistence of tobacco-smoke particles in the alveolar space, which could influence cell activity.
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References
Greening, A. 1991. Pulmonary disorders.In Clinical Immunology. J. Brostoff, G. K. Scadding, D. Male, and I. M. Roitt, editors. Gower Medical Publishing, London, pp. 12.1–12.16.
Janoff, A. 1988. Emphysema: Proteinase-antiproteinase imbalance.In Inflammation: Basic Principles and Clinical Correlates. J. I. Gallin, I. M. Goldstein, and R. Snyderman, editors. Raven Press, New York. 803–914.
Snider, G. L. 1988. Interstitial lung diseases: Pathogenesis, pathophysiology, and clinical presentation.In Interstitial Lung Diseases. M. I. Schwarz, and T. E. King, editors. B. C. Decker, Toronto. 1–14.
Holt, P. G. 1987. Immune and inflammatory function in cigarette smokers.Thorax 42:241–249.
Ando, M., M. Sugimoto, R. Nishi, M. Suga, S. Horio, H. Kohrogi, K. Shimazu, andS. Araki. 1987. Surface morphology and function of human pulmonary alveolar macrophages from smokers and nonsmokers.Thorax 39:850–856.
Capelli, A., M. Lusuardi, S. Carli, andC. F. Donner. 1991. Acid phosphatase(EC3.1.3.2) activity in alveolar macrophages from patients with active sarcoidosis.Chest 99:545–550.
Chang, J. C., O. H. Yoo, andM. Lesser. 1989. Cathepsin D activity is increased in alveolar macrophages and bronchoalveolar lavage fluid of smokers.Am. Rev. Respir. Dis. 140:958–960.
Nakashima, H., M. Ando, M. Sugimoto, M. Suga, K. Soda, andS. Araki. 1987. Receptormediated and O −2 release by alveolar macrophages and peripheral blood monocytes from smokers and nonsmokers.Am. Rev. Respir. Dis. 136:310–315.
Thomassen, M. J., B. P. Barna, H. P. Weidemann, M. Farmer, M. Ahmad.1988.Human.J. Leuk. Biol. 44:313–318.
Sköld, C. M., J. Hed, andA. Eklund. 1992. Smoking cessation rapidly reduces cell recovery in bronchoalveolar lavage fluid, while alveolar macrophage fluorescence remains high.Chest 101:989–995.
Eklund, A., andE. Blaschke. 1986. Relationship between changed alveolar-capillary permeability and angiotensin-converting enzyme in serum in sarcoidosis.Thorax 41:629–634.
Pedersen, B., R. Dahl, E. Juel Jensen. 1988. Bronchoalveolar lavage and activity of alveolar macrophages in patients with bronchial hyperreactivity before and after smoking cessation.Allergy 43(suppl. 7)33 (abstract).
Sköld, C. M., A. Eklund, G. Halldén, andJ. Hed. 1989. Autofluorescence in alveolar macrophages from smokers; relation to cell surface markers and phagocytosis.Exp. Lung Res. 15:823–835.
Sköld, C. M., A. Eklund, J. Hed, andR. Hernbrand. 1992. Endocytosis of cigarette-smoke condensate by rabbit alveolar macrophages in vitro measured as fluorescence intensity.Eur. Respir. J. 5:53–58.
Hillerdal, G., andR. Rylander. 1984. Asthma and cessation of smoking.Clin. Allergy 14:45–47.
Fennerty, A. G., J. Banks, P. Ebden, andC. Bevan. 1987. The effects of cigarette withdrawal on asthmatics who smoke.Eur. J. Respir. Dis. 71:395–399.
Mullen, J. B. M., J. L. Wright, B. R. Wiggs, P. D. Paré, andJ. C. Hogg. 1987. Structure of central airways in current smokers and ex-smokers with and without mucus hypersecretion: Relationship to lung function.Thorax 42:843–848.
Camner, P. 1980. Clearance of particles from the human tracheobronchial tree.Clin. Sci. 59:79–84.
Camner, P., K. Philipson, andT. Arvidsson. 1973. Withdrawal of cigarette smoking. A study on tracheobronchial clearance.Arch. Environ. Health 26:90–92.
Hoidal, J. R., R. B. Fox, P. A. LeMarbe, R. Perri, andJ. E. Repine. 1981. Altered oxidative metabolic responses in vitro of alveolar macrophages from asymptomatic cigarette smokers.Am. Rev. Respir. Dis. 123:85–89.
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Sköld, C.M., Forslid, J., Eklund, A. et al. Metabolic activity in human alveolar macrophages increases after cessation of smoking. Inflammation 17, 345–352 (1993). https://doi.org/10.1007/BF00918995
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DOI: https://doi.org/10.1007/BF00918995