Supplementary Material
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Supplementary material. ERJ-01458-2019.SUPPLEMENT
Supplementary figure E1. a) The effect of ATP on contraction of primary HASM cells alone. ATP at 0.3, 1 or 3 mM did not cause contraction of the cells analysed using ptychography, in some cases there was relaxation. The contractile stimulus ACh (10 μM) caused contraction of the cells as expected. Data shown as % change from baseline (resting state), N=2 patients, n=17–27 cells. b) The effect of vehicle, ATP at 1 mM and 3 mM and PMA (10 nM) ionomycin (1 μM) on total cysteinyl leukotriene release from primary human mast cells. Addition of ATP (1 or 3 nM) caused a concentration dependent increase in total cysteinyl leukotriene release compared to vehicle control. Data shown is from mast cells isolated from 1 (73-year-old female donor). ERJ-01458-2019.FIGURE_E1
Supplementary figure E2. a) PAR2 mRNA was expressed in HASMCs from six patients, but not in HLMCs of two patients, and expressed at very low levels in the third. The effect of vehicle GSK2193874, montelukast and 5BDBD on contraction induced by the common contractile stimuli histamine (b) and acetylcholine (c) in isolated guinea pig trachea. No antagonist had any effect on contraction different to that of vehicle. Mean±SEM of n=6 (HASMs), 3 (HLMCs), 3–4 (trachea). ERJ-01458-2019.FIGURE_E2
Supplementary video. ERJ-01458-2019.VIDEO