Table 3– Threshold cycle difference (δCT) values for cytokine genes after 24 h of in vitro culture in the presence or absence of heat-killed Mycobaterium tuberculosis
mRNAUnstimulatedStimulatedUnstimulated versus stimulated p-value
IRISNon-IRISp-valueIRISNon-IRISp-valueIRISnon-IRIS
IL-1β6.4 (-1.3–7.8)6.7 (4.0–8.6)0.202-2.7 (-3.6–1.7)-0.6 (-1.9–0.2)<0.001<0.001<0.001
IL-211.5 (9.0–12.9)12.7 (12.2–13.7)0.0148.3 (7.5–9.4)10.0 (7.0–12.7)0.129<0.001<0.001
IL-415.5 (14.9–16.7)16.1 (15.3–17.1)0.40914.4 (14.1–15.8)15.4 (14.0–16.6)0.3820.0010.004
IL-520.2 (19.3–20.6)18.9 (18.0–20.0)0.00312.1 (11.4–14.7)17.0 (13.9–20.1)0.001<0.0010.13
IL-612.0 (4.0–13.8)12.4 (9.7–13.9)0.781.3 (0.3–3.7)4.6 (3.1–5.9)<0.001<0.001<0.001
IL-83.0 (-0.2–3.8)2.6 (0.4–5.1)0.48-3.7 (-4.6–2.1)-1.5 (-2.8–1.6)0.006<0.0010.002
IL-106.9 (5.1–8.1)6.9 (6.0–7.1)0.744.9 (3.1–6.4)6.8 (5.9–8.5)<0.001<0.0011.0
IL-12p4016.2 (12.1–17.5)16.9 (13.9–18.1)
0.208.1 (6.5–9.4)10.0 (7.8–13.3)0.005<0.001<0.001
IL-1317.6 (13.5–10.3)17.6 (14.8–18.4)0.618.8 (7.9–10.0)11.0 (9.4–13.7)0.002<0.001<0.001
IL-159.6 (8.6–10.5)10.6 (10.1–11.4)0.0058.4 (7.9–8.7)8.6 (8.0–9.6)0.140.004<0.001
IL-17A18.3 (16.3–19.5)18.2 (17.7–19.4)0.69812.7 (11.5–14.1)13.9 (12.8–15.3)0.048<0.001<0.001
IL-189.9 (9.2–10.4)10.5 (9.0–12.6)0.149.9 (8.9–10.7)9.6 (8.6–11.4)0.850.450.36
IFN−γ9.4 (6.0–10.6)9.6 (8.6–10.7)0.2593.3 (1.8–5.1)6.8 (4.3–8.1)0.002<0.001<0.001
GM-CSF11.8 (8.6–14.3)13.6 (10.8–15.0)0.3822.8 (-0.9–3.7)5.0 (3.9–6.8)<0.001<0.001<0.001
TGF-β4.7 (4.4–5.4)4.5 (4.1–4.9)0.14.6 (4.3–4.9)4.8 (4.5–5.6)0.160.180.004
TNF7.3 (2.7–7.90)8.2 (7.7–8.5)<0.0011.4 (1.1–2.0)3.2 (2.4–4.9)<0.001<0.0010.002

  • IRIS: immune reconstitution inflammatory syndrome; IL: interleukin; IFN: interferon; GM-CSF: granulocyte-macrophage colony-stimulating factor; TGF: tumour growth factor; TNF: tumour necrosis factor. The p-values are uncorrected for multiple comparisons in the table but multiple comparisons were factored in the analysis (see text). Lower values indicate high transcript abundance of the gene of interest.