| Inoculum CFU | ||
| 3×103 | 1×104 | |
| KC pg·mL−1 | ||
| WT | 4150±477 | 6360±667 |
| TLR2 KO | 3688±346 | 5490±432 |
| TLR4 KO | 3261±698 | 3982±1023 |
| TLR2×4 KO | 2035±471 | 4459±1056 |
| IL-1β pg·mL−1 | ||
| WT | 2401±309 | 1936±391 |
| TLR2 KO | 3842±620* | 2504±302 |
| TLR4 KO | 1549±436 | 799±301 |
| TLR2×4 KO | 781±580*** | 1763±580 |
| TNF pg·mL−1 | ||
| WT | 188±18 | 965±103 |
| TLR2 KO | 256±29 | 1551±341 |
| TLR4 KO | 146±13* | 650±130 |
| TLR2×4 KO | 129±2** | 719±147 |
| MIP-2 | ||
| WT | 4290±1308 | 12782±3566 |
| TLR2 KO | 6132±848 | 32579±1671** |
| TLR4 KO | 3137±862 | 6466±2831 |
| TLR2×4 KO | 1717±526 | 17088±5739 |
| IL-6 | ||
| WT | 1602±325 | 1528±480 |
| TLR2 KO | 2934±580 | 3355±776 |
| TLR4 KO | 1964±445 | 1637±667 |
| TLR2×4 KO | 1452±319 | 3693±1473 |
| Histopathological score | ||
| WT | 7.3±0.6 | 10.1±1.2 |
| TLR2 KO | 8.5±0.6 | 10.5±0.4 |
| TLR4 KO | 6.2±0.9 | 7.9±1.0 |
| TLR2×4 KO | 4.6±0.7** | 9.4±1.1 |
Data are presented as mean±sem. Wild-type (WT), Toll-like receptor (TLR)2, TLR4 and TLR2×4 knockout (KO) mice were infected with the indicated inocula of Klebsiella pneumoniae. After 24 h, mice were sacrificed, right lungs were removed and keratinocyte-derived chemokine (KC), interleukin (IL)-1β, tumour necrosis factor (TNF), macrophage inflammatory protein (MIP)-2 and IL-6 were determined using ELISA. Left lungs were used for determining histopathology scores as described in the Methods section. n = 7–8 mice per group. *: p<0.05 versus WT; **: p<0.01 versus WT; ***: p<0.001 versus WT.